| TCA-丙酮沉淀法提取聚合草叶蛋白的研究 |
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| 引用本文： | 董双涛,李宝霞,霍乃蕊.TCA-丙酮沉淀法提取聚合草叶蛋白的研究[J].世界中西医结合杂志,2011,6(9):766-767,828. |
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| 作者姓名： | 董双涛 李宝霞 霍乃蕊 |
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| 作者单位： | 1. 山西生物应用职业技术学院,山西,太原,030031
2. 山西农业大学食品科学与工程学院,山西,太谷,030801 |
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| 基金项目： | 山西省科技攻关项目，山西省回国留学人员科研项目 |
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| 摘 要： | 目的本研究旨在用TCA-丙酮沉淀法对聚合草叶蛋白进行提取,为聚合草叶蛋白的大规模制备奠定基础。方法测定聚合草干叶、鲜叶中的蛋白含量,提取的蛋白经真空冷冻干燥后称重,确定所提蛋白的纯度并计算蛋白提取率。结果以鲜叶为原料时的提取率为51.76%,所得蛋白的纯度为54.2%;以干叶为原料时,蛋白提取率为46.45%,蛋白纯度为48.3%。结论利用TCA-丙酮沉淀法从聚合草鲜叶中提取粗蛋白优于干叶。
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| 关 键 词： | 聚合草 叶蛋白 TCA-丙酮沉淀法 |
Study on the Extraction of Leaf Protein of Symphytum Pezegrinum L.with TCA-Acetone Precipitation |
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| DONG Shuang-tao,LI Bao-xia,HUO Nai-rui.Study on the Extraction of Leaf Protein of Symphytum Pezegrinum L.with TCA-Acetone Precipitation[J].World Journal Of Integrated Traditional and Wesrern Medicine,2011,6(9):766-767,828. |
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| Authors: | DONG Shuang-tao LI Bao-xia HUO Nai-rui |
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| Institution: | DONG Shuang-tao1,LI Bao-xia1,HUO Nai-rui2(1.Shanxi Vocational and Technical College of Biological Application,Taiyuan Shanxi 030031,2.Food Science and Engineering College,Shanxi Agricultural University,Taigu Shanxi 030801) |
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| Abstract: | Objective To study the extraction of leaf protein of Symphytum Pezegrinum L.with TCA-acetone precipitation so as to lay the foundation for the large-scale preparation of Symphytum Pezegrinum L.Methods Detecting the protein contents in dry leaf and fresh leaf of Symphytum Pezegrinum L.,weighting the extracted protein after vacuum freezing-drying and determining the purity of the extracted protein and calculating protein extraction rate.Results The extraction rate of fresh leaf was 51.76% and the protein puri... |
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| Keywords: | Symphytum Pezegrinum L Leaf protein TCA-acetone precipitation |
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