肾综合征出血热可溶性免疫复合物的致病变作用

目的确定可溶性免疫复合物在肾综合征出血热（ＨＦＲＳ）发病中的作用。方法预先在体外制备流行性出血热病毒抗原特异的可溶性免疫复合物,经尾静脉注入正常ＢＡＬＢ／ｃ小鼠体内,用免疫组织化学ＡＢＣ法,对免疫复合物（ＩＣ）在小鼠主要脏器的组织分布进行定位。结果注射ＩＣ后的２４小时,ＩＣ主要见于血管系统如肝血窦,脾血窦,肠道固有层和粘膜下层的血管,肾小球毛细血管等。从４８小时开始,ＩＣ出现于器官实质细胞如肝细胞,肾小管上皮细胞以及某些腺体的导管上皮细胞（如颌下腺,胰腺等）。ＩＣ存在部位的细胞出现变性改变,以肝细胞,肾小管上皮细胞最为明显,这些病变与流行性出血热病人组织的病变极其相似。而注射非特异性ＩＣ的动物则无这种病变。结论在ＨＦＲＳ的发病中,ＨＦＲＳ的可溶性ＩＣ可直接引起组织损伤。

Effect of the soluble immunocomplexes on the pathogenesis of hemorrhagic fever with renal syndrome

OBJECTIVE: To determine the effect of the soluble immunocomplexes on the pathogenesis of hemorrhagic fever with renal syndrome (HFRS). METHODS: The soluble immunocomplex (IC) consisting of HFRS virus antigen and antibody was prepared previously in vitro, and was intravenously injected into BALB/c mice. The localization of IC in the tissues of mice was investigated by immunohistochemical method ABC. RESULTS: The results showed that 24 hours after injection, ICs were localized mostly in the blood vessels, such as the liver sinusoids, the splenic sinuses, the blood vessels of the mucosa and submucosa of the intestine, and the capillaries of the renal glomeruli, etc. 48 hours later or more, IC localized primarily on the parachymal cells, e.g., the hepatocytes, the epithelial cells of the renal tubules as well as some epithelial cells of the ducts and alveoli of some glands (submandular glands, pancreas). The cells degenerated in the areas with IC, especially in the hepatocytes and epithelial cells of renal tubules. These lesions were very similar to the pathological changes of the HFRS' patients, and were not found in patients injected with other IC. CONCLUSION: The soluble IC of HFRS can cause direct tissue injury in the HFRS.