维生素C干扰血糖测定的探讨

维生素C(以下简称Vc)干扰血糖测定,造成测定值的负偏差。当血液中Vc达到一定浓度时,可以使血糖测定的反应不显色,测定结果为零,甚至出现负值。用氯化汞(HgCl2)络合Vc,将络合物离心沉淀,用上清测血糖。经反复试验,从反应条件到试剂用量,总结出一套较完善的测定方法。由于氯化汞用量甚微,加入量对原血清的稀释作用可忽略不计。由该方法测定经氯化汞络合Vc后的血糖值,与加Vc前的原始血糖值无显著性差异,而且该方法具有简便、快速、准确等优点,能为临床一部分特殊病人的诊断、治疗提供可靠依据。

Study of Vitamin C Interference in Serum Glucose Determination

In the clinical laboratory,Vitamin C is one of the most important factors interfering with serum glucose determination.A negative value may be observed due to the Vitamin C interference.We use HgCl2 to precipitate the Vitamin C,and the serum glucose can be accurately detected in the supernatant after centrifugation,so the disruption of Vitamin C is removed.We can conclude that the method is simple,quick and accurate.It can do much favor of the clinic test for some special patients.