IL-6基因调控区-174G/C多态性与急性脑梗死的关系

目的　探讨急性脑梗死患者 IL - 6基因调控区 - 174位点多态性与疾病的相关关系。方法　急性脑梗死患者 42例 ,男 2 4例 ,女 18例 ,年龄 41～ 90岁 ,平均 6 6 .6± 9.8岁。健康对照组 18例 ,男 11例 ,女 7例 ,年龄 31～ 5 4岁 ,平均 45 .6± 8.7岁。采用 PCR扩增、限制性内切酶片段长度多态性 ( RFL P)分析和 DNA序列测定 ,认识IL - 6基因调控区 - 174位点的多态性 ;同时 EL ISA定量分析脑梗死患者和正常人血清白细胞介素 - 6的含量。结果　急性脑梗死患者 IL - 6基因调控区 - 174位点均为 GG型。患者血清白细胞介素 - 6水平升高 ,明显高与正常对照组 ( P<0 .0 1)。结论　急性脑梗死患者未发现 IL - 6基因调控区 - 174位点的基因变异 ,推测 - 174位点的多态性没有参与急性脑梗死的发生和发展过程。

Relationship between G to C polymorphism at -174 of the interleukin-6 gene and acute cerebral infarction

Objective To elucidate the relationship between the G/C polymorphism at-174 of the interleukin-6 gene and acute cerebral infarction.Methods Objects examined were composed of two groups: 42 patients with cerebral infarction and 18 healthy controls. Adopt PCR, RFLP and sequence analysis to know the G/C polymorphism at-174 of the interleukin-6 gene in patients with cerebral infarction.Levels of circulating IL-6 were analysed by ELISA in 42 patients with cerebral infarction and in 18 healthy controls. Results The -174 genotype of the interleukin-6 gene in 42 patients with cerebral infarction was normal GG. Plasma IL-6 was elevated in these patients compared with healthy controls ( P <0.01). Conclusion The study suggestes that G to C variation at-174 of the interleukin-6 gene is not associated with the cerebral infarction.