半夏药材中蛋白质IEF电泳图谱的研究

目的通过对半夏及其近缘植物的蛋白质电泳图谱比较,研究半夏药材的电泳鉴别方法。方法应用等电聚焦(isoe lectrofocus ing,IEF)技术,对不同产地半夏及其近缘植物块茎进行比较。结果半夏具有稳定的IEF电泳图谱,显示具有8条特征蛋白质带,并测定了其等电点(P I)值,半夏与其几种近缘植物的IEF谱有明显的区别。结论半夏IEF电泳图谱可作为半夏药材的鉴别依据,半夏特征蛋白质的P I值为半夏的鉴定和蛋白质分离纯化提供了参数。

IEF Electrophorogram of protein in Rhizoma Pinelliae

Objective To discriminate Rhizoma Pinelliae from its relative species by electrophoro-gram of protein.Methods Using isoelectrofocusing(IEF) technology to compare the electrophorogram of tuber of Pinellia ternata produced in different habitats and from its relative plants then determining the pH values.Results The IEF electrophorogram of Rhizoma Pinelliae was stable with eight clear characteristic protein bands and had obvious differences from the electrophorogram of the tubers of its relative plants.Conclusion The IEF electrophoresis of Rhizoma Pinelliae can be used as the distinguishing basis.The PI value of the characteristic protein of P.ternana offers the basic parameter for separation and purification of the protein in Rhizoma Pinelliae.