| 肺成纤维细胞对裸鼠肺癌转移影响及其机制的探讨 |
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| 引用本文： | 朱运奎,杨娜,闫江涛,李继东,张冬泉,范红燕.肺成纤维细胞对裸鼠肺癌转移影响及其机制的探讨[J].中华肿瘤防治杂志,2011,18(20):1590-1593,1607. |
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| 作者姓名： | 朱运奎 杨娜 闫江涛 李继东 张冬泉 范红燕 |
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| 作者单位： | 中国人民解放军兰州军区总医院呼吸内科,甘肃兰州,730005 |
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| 摘 要： | 目的:探讨成纤维细胞在裸鼠肺癌生长和转移过程中的作用。方法:将裸鼠40只随机分成实验组和对照组两组,将H460细胞、H460细胞+成纤维细胞分别接种裸鼠左侧胸腔,观察两组裸鼠共3周。统计其生存率,测量瘤质量,蛋白质印迹法测定动物肿瘤组织内基质金属蛋白酶1(MMP-1)的表达水平,明胶酶普法检测血清中基质金属蛋白酶-2(MMP-2)的表达。结果:裸鼠肺癌胸腔内接植成瘤率为100%,胸腔种植瘤模型逐渐出现远处转移。对照组于第18、19、20和21天共有4只裸鼠死亡,实验组分别于第12、15和19天共有3只死亡,第18、20天各有2只死亡,第21天有3只死亡,差异有统计学意义,P=0.004 8;实验组裸鼠死亡时间均较对照组早。实验组瘤质量(1.711±0.175)高于对照组(1.521±0.369),P=0.000。实验组动物血液中MMP-2的表达及瘤组织内MMP-1表达水平明显高于对照组,P<0.05。结论:成纤维细胞能够促进裸鼠肺癌的生长和转移,其作用机制可能是同肿瘤细胞相互协同,促进裸鼠体内MMP-1、MMP-2的表达有关。
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| 关 键 词： | 肺肿瘤 细胞培养 基质金属蛋白酶类 |
Effects of fibroblast cells on tumor growth and metastasis of lung cancer in athymic mouse |
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| ZHU Yun-kui,YANG Na,YAN Jiang-tao,LI Ji-dong,ZHANG Dong-quan,FAN Hong-yan.Effects of fibroblast cells on tumor growth and metastasis of lung cancer in athymic mouse[J].Chinese Journal of Cancer Prevention and Treatment,2011,18(20):1590-1593,1607. |
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| Authors: | ZHU Yun-kui YANG Na YAN Jiang-tao LI Ji-dong ZHANG Dong-quan FAN Hong-yan |
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| Institution: | ZHU Yun-kui,YANG Na,YAN Jiang-tao,LI Ji-dong,ZHANG Dong-quan,FAN Hong-yan Department of Respiratory Medicine,Lanzhou General Hospital of Lanzhou Military Area Command of PLA,Lanzhou 730005,P.R.China |
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| Abstract: | OBJECTIVE:To evaluate the effects of human fetal lung fibroblasts(HFL) cells on tumor growth and metastasis of lung cancer in athymic mouse.METHODS: Forty athymic mouses were randomized into experimental group and gelatinase zymography control group,H460 cells along and H460 with HFL were inoculated into the thoracic cavity of athymic mouse.After three weeks,calculated the transplantation tumor weights and mice life span,meanwhile,MMP-2 in the blood gelatinase zymography of the mouses were detected by gelat... |
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| Keywords: | lung neoplasms cell culture matrix metalloproteinases |
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