| 蛋白芯片技术及免疫印迹技术检测抗核抗体的比对研究 |
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| 引用本文： | 江凌晓,张德明,林丽娟,姜长宏,方艳萍. 蛋白芯片技术及免疫印迹技术检测抗核抗体的比对研究[J]. 分子诊断与治疗杂志, 2011, 3(6): 413-415 |
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| 作者姓名： | 江凌晓 张德明 林丽娟 姜长宏 方艳萍 |
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| 作者单位： | 南方医科大学珠江医院医学检验中心,广东,广州510282 |
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| 摘 要： | 目的 通过比对免疫印迹法与蛋白芯片法对血清标本中抗核抗体的检测结果,验证这两种方法是否具有等效性. 方法 分别用免疫印迹技术和蛋白芯片技术检测70例临床标本的抗核抗体,记录检验结果,并对检查结果进行统计学分析. 结果 经配对资料卡方检验分析,两种方法测定SSA、SSB、SM、Scl-70、Jo-1和CENP时结果差异无...
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| 关 键 词： | 蛋白芯片法 免疫印迹法 抗核抗体 |
Comparison of immunoblot and protein chip in detection of antinuclear antibodies |
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| JIANG Lingxiao,ZHANG Deming,LIN Lijuan,JIANG Changhong,FANG Yanping. Comparison of immunoblot and protein chip in detection of antinuclear antibodies[J]. Journal of Molecular Diagnosis and Therapy, 2011, 3(6): 413-415 |
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| Authors: | JIANG Lingxiao ZHANG Deming LIN Lijuan JIANG Changhong FANG Yanping |
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| Affiliation: | (Center of Clinical Laboratory, Zhujiang Hospital, Guangdong, Guangzhou 510282, China) |
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| Abstract: | Objective To compare the results of immunoblot assay and protein chip technique in detecting antinuclear antibodies（ANA） of human serum in order to testify if these two methods have equivalency. Methods 70 serum samples with ANA were detected by immunoblotting and protein chip technique respectively. Then werecorded the results of experiments and analyzed them by approcriate statistical method. Results No significant statistical difference was found between the results of these two methods in detecting SSA, SSB, SM, Scl-70, Jo-1 and CENP（P〉0.05） while it was were only observed in RNP test （P=0.04）. Conclusion The results of these two methods in detecting ANA had good consistency and protein chip techinique can be applied to clinical ANA tests. |
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| Keywords: | Protein chip technique Immunoblot assay Antinuclear antibodies |
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