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| 南中国多棘海星乙醇提取物体外抗肿瘤活性作用的初步研究 | |
| 引用本文： | 何素辉,唐晓磊,侯玉涛,邓亦峰,陈章权.南中国多棘海星乙醇提取物体外抗肿瘤活性作用的初步研究[J].分子诊断与治疗杂志,2011,3(6):402-405. |
| 作者姓名： | 何素辉 唐晓磊 侯玉涛 邓亦峰 陈章权 |
| 作者单位： | 1. 广东省医学分子诊断重点实验室,广东医学院,广东,东莞523808;广东医学院门诊部,广东,东莞523808 2. 广东省医学分子诊断重点实验室,广东医学院,广东,东莞523808 3. 广东省天然药物研究与开发重点实验室,广东医学院,广东,湛江524023 |
| 基金项目： | 东莞市高等院校科研机构科技计划项目（2008108101041） |
| 摘 要： | 目的 研究多棘海星乙醇提取物对人肿瘤细胞体外生长的影响,为进一步筛选抗肿瘤活性成分提供重要的科学依据. 方法 用硅胶柱层析法对多棘海星乙醇提取物进行组分分离,采用MTT法观察各个组分对体外培养的人乳腺癌细胞MDA-MB-231、人宫颈癌Hela细胞、鼻咽癌CNE-1细胞等肿瘤细胞株增殖的抑制作用.结果 通过硅胶柱层析从... |
| 关 键 词： | 海星 乙醇提取物 抗肿瘤 |
Primary study on the antitumor effect of ethanol extracts of starfish Asterias amurensis |
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| HE Suhui,TANG Xiaolei,HOU Yutao,DENG Yifeng,CHEN Zhangquan.Primary study on the antitumor effect of ethanol extracts of starfish Asterias amurensis[J].Journal of Molecular Diagnosis and Therapy,2011,3(6):402-405. | |
| Authors: | HE Suhui TANG Xiaolei HOU Yutao DENG Yifeng CHEN Zhangquan |
| Institution: | 1. Guangdong Provincial Key Laboratory for Medical Molecular Diagnostics, Guangdong Medical College, Guangdong, Dongguan 523808, China; 2. Out-patient Department, Guangdong Medical College, Guangdong, Dongguan 523808, China; 3. Guangdong Provincial Key Laboratory for Research and Development of Natural Drugs, Guangdong Medical College, Guangdong, Zhanjiang 524023, China) |
| Abstract: | Objective To investigate the antitumor activity of the ethanol extracts of starfish Asterias amurensis in vitro. Methods Silica gel column chromatography was used to separate the fractions of the ethanol extracts of starfish Asterias amurensis. In vitro, the inhibitory effects of ethanol extracts of starfish Asterias amurensis on proliferation of human breast carcinoma cell （MDA-MB-231）, cervical cancer cell line （Hela Cell） and nasopharyngeal carcinoma cell line（CNE-1） were measured by MTT colormetric assay, Results The ethanol extracts from starfish were separated in silica gel column to obtain 8 fractions （I-VIII）, 4 of which could inhibit carcinoma cells proliferation. The fraction IV and VIII could significantly inhibit the proliferation of MDA- MB-231, Hela and CNE-1 cells （P〈0.01）, fraction V also could inhibit the proliferation of Hela cell （P〈0.05）, and fraction VII showed the inhibitory effects on proliferation of Hela （P〈0.01）, while the other 4 fractions of I, II, III and VI did not show the inhibitory effects on carcinoma cell proliferation（P〉0.05）, Conclusion The ethanol extracts of starfish Asterias amurensis possess significant inhibitory effect on carcinoma cells, and the fractions IV and VIII may be the major fraction contains antitumor active substance. |
| Keywords: | Starfish Ethanol extract Antitumor |
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