原发性肾病综合征湿热证与促炎症因子的相关性研究

目的:探讨原发性肾病综合征(PNS)湿热证与促炎症因子的相关性。方法:采用前瞻、随机对照的临床研究方法,选择PNS湿热证40例和非湿热证20例,观察两组患者血清超敏CRP(hs-CRP)、白细胞介素-6(IL-6)、核因子-κB(NF-κB),尿单核细胞趋化蛋白-1(MCP-1)含量的差异;再将湿热证组随机分为对照组和黄葵胶囊治疗组各20例,比较两组治疗前后湿热证候与促炎症因子水平的变化情况。结果:湿热证组hs-CRP、MCP-1、IL-6、NF-κB含量明显高于非湿热证组(P<0.05,P<0.01);湿热证对照组和治疗组治疗后证候积分及促炎症因子水平均较治疗前明显下降(P<0.05,P<0.01),但治疗组下降更显著(P<0.05,P<0.01),湿热证证候积分与NF-κB、IL-6、MCP-1含量呈正相关(r分别为0.52,0.53,0.42,均P<0.01)。结论:湿热因素可促进PNS hs-CRP、IL-6、MCP-1、NF-κB的表达,PNS湿热证与促炎症因子水平升高存在一定相关性。

The Correlation Study Between Damp-heat Syndrome and Pro-inflammatory Cytokines In Primary Nephrotic Syndrome

Objective：To observe the correlation between PNS Damp-heat syndrome and pro-inflammatory cytokines.Methods：Using prospective,randomized controlled clinical research methods,we selected 40 Damp-heat syndrome cases and 20 non Damp-heat syndrome cases,observed the difference between the two groups in serum hs-CRP,IL-6,NF-κB and urine MCP-1 levels;again the Damp-heat syndrome group was randomly divided into control group and Huangkui Capsule treatment group,20 patients in each,compared the changes of pro-inflammatory cytokines and Damp-heat syndrome before and after treatment.Results：hs-CRP,MCP-1,IL-6 and NF-κB activity in Damp-heat syndrome group were significantly higher than nondamp-heat syndrome group（P0.05,P0.01）.After treatment,Damp-heat symptom scores and content of pro-inflammatory cytokines in Damp-heat treatment group and control group were decreased compared with treatment before,but in treatment group the decreased was obvious（P0.05,P0.01）.Damp-heat symptom scores was positively correlated with NF-κB,IL-6 and MCP-1 content（r =0.52,0.53,0.42 respectively,all P0.01）.Conclusion：Damp-heat factor increase the expression of hs-CRP、IL-6、MCP-1、NF-κB,PNS Damp-heat syndrome is closely related to pro-inflammatory cytokines.