羟喜树碱对黑素瘤B16F10细胞侵袭和凋亡的影响及作用机制

目的:观察羟喜树碱(hydroxycamptothecin,HCPT)对黑素瘤B16F10细胞侵袭能力以及凋亡相关蛋白p53和Bcl-2表达的影响,并探讨其抑制黑素瘤细胞的作用机制.方法:分别用5、10、20、50μmol/L的HCPT处理B16F10细胞24 h及48 h,采用Transwell实验评价细胞的侵袭能力...

Effects and mechanism of hydroxycamptothecin on the invasion and apoptosis of melanoma B16F10 cells

Objective:To observe the effects of hydroxycamptothecin(HCPT)on the invasion of melanoma B16F10 cells and the expression of apoptosis-related proteins p53 and Bcl-2,and also to explore possible mechanism of the inhibition of melanoma cells.Methods:Melanoma B16F10 cells were respectively treated with 5,10,20 and 50μmol/L HCPT for 24 and 48 hours.Cell migration was assessed by Transwell assay and protein expression and mRNA expression of p53 and Bcl-2 were detected by Western blot and RT-PCR.Results:Transwell results showed that the in vitro invasion of B16F10 cells significantly decreased in different HCPT concentrations groups(except in 5μmol/L concentration group),as compared with the blank group(P<0.01).In addition,HCPT could significantly increase the mRNA and protein level of p53,decrease the mRNA and protein level of Bcl-2(P<0.01),and at the same time presented a trend of obvious dose-dependence and time-dependence.Conclusion:HCPT could significantly reduce the invasion of B16F10 cells in vitro and influence the expression of p53 and Bcl-2 in B16F10 cells,thus promoting the apoptosis of tumor cells.