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HPLC一测多评法同时测定胃疡灵颗粒中8种成分的含量
引用本文:邵士川,孙慧清,许伟,马灵珍. HPLC一测多评法同时测定胃疡灵颗粒中8种成分的含量[J]. 药学服务与研究, 2022, 0(1): 34-38,44
作者姓名:邵士川  孙慧清  许伟  马灵珍
作者单位:泰安市中心医院药学部;东营市食品药品检验中心药品科;亳州职业技术学院药学院
基金项目:安徽省教育厅高等学校省级质量工程项目(2018ylzy164)。
摘    要:目的:建立HPLC一测多评法同时测定胃疡灵颗粒中肉桂酸、桂皮醛、毛蕊异黄酮葡萄糖苷、芒柄花苷、芒柄花素、6-姜辣素、8-姜酚和10-姜酚的含量.方法:采用HPLC法,以Agilent SB-C18柱(250 mm×4.6 mm,5μm)为色谱柱,流动相A为乙腈-甲醇(9:1),流动相B为0.1% 磷酸溶液,梯度洗脱(0...

关 键 词:胃疡灵颗粒  含量测定  色谱法  高效液相  一测多评法  相对校正因子  毛蕊异黄酮葡萄糖苷

Simultaneous determination of eight components in Weiyangling granules by HPLC-quantitative analysis of multi-components by single marker
SHAO Shichuan,SUN Huiqing,XU Wei,MA Lingzhen. Simultaneous determination of eight components in Weiyangling granules by HPLC-quantitative analysis of multi-components by single marker[J]. Pharmaceutical Care and Research, 2022, 0(1): 34-38,44
Authors:SHAO Shichuan  SUN Huiqing  XU Wei  MA Lingzhen
Affiliation:(Department of Pharmacy,Taian City Cental Hospital,Shandong Taian 271000,China;Department of Drugs,Dongying Food and Drug Inspection Center,Shandong Dongying 257091,China;School of Pharmacy,Bozhou Vocational and Technical College,Anhui Bozhou 236800,China)
Abstract:Objective:To establish a high performance liquid chromatography quantitative analysis of multi-components by single marker(HPLC-QAMS)for simultaneous determination of cinnamic acid,cinnamaldehyde,calycosin-7-O-β-D-glucopyranoside,ononin,formononetin,6-gingerol,8-gingerol and 10-gingerol in Weiyangling granules.Methods:An HPLC method was established with Agilent SB-C18(250 mm×4.6 mm,5μm)as a separate column.Acetonitrile-methanol(9∶1,phase A)and 0.1%phosphoric acid(phase B)were used as a mobile phase,and the flow rate was 1.1 ml/min with a gradient elution(0-13 min,28%A;13-24 min,28%→35%A;24-39 min,35%→56%A;39-53 min,56%→85%A;53-60 min,85%→28%A).The detection wavelength was set at 280 nm and the column temperature was 25℃.With calycosin-7-O-β-D-glucopyranoside as an internal standard,the relative correction factors of the other seven components were established and the contents of various components were calculated.Results:Cinnamic acid,cinnamaldehyde,calycosi-7-O-β-D-glucopyranoside,ononin,formononetin,6-gingerol,8-gingerol and 10-gingerol showed good linear relationships within the ranges of 0.59-11.80,7.47-149.40,2.18-43.60,1.91-38.20,4.49-89.80,3.87-77.40,0.94-18.80 and 1.46-29.20μg/ml(r≥0.9991),with average recovery rates of(96.89±1.04)%,(100.05±0.64)%,(99.52±1.18)%,(98.51±1.30)%,(99.38±0.77)%,(98.29±0.88)%,(97.72±1.26)%and(97.93±1.01)%(n=9)respectively.The relative correction factors of cinnamic acid,cinnamaldehyde,ononin,formononetin,6-gingerol,8-gingerol and 10-gingerol were respectively 0.8263,0.8490,0.9720,1.2005,1.2766,0.7202 and 0.8749.There were no significant differences between the calculated values by HPLC-QAMS method and the measured values by external standard method.Conclusion:The HPLC-QAMS method established by our study is simple and accurate,which could be used for the quality control of Weiyangling granules.
Keywords:Weiyangling granule  content determination  chromatography,high performance liquid  quantitative analysis of multi-components by single marker  relative correction factor  calycosin-7-O-β-D-glucopyranoside
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