兔动脉粥样硬化模型中NO和PAI-1的表达及阿托伐他汀的干预作用

目的： 探讨兔动脉粥样硬化模型中一氧化氮（NO）和血浆纤溶酶原激活物抑制物-1（PAI-1）的表达及阿托伐他汀的干预作用。方法：24只雄性日本大耳白兔随机分为高脂饮食组(A组)、高脂饮食+阿托伐他汀组(B组)和正常饮食组(C组)，每组8只。各组分别在喂养16周末处死， ELISA法检测NO和PAI-1。全自动生化分析仪检测血清脂蛋白的表达水平。结果：NO的表达水平B组显著高于A组和C组（P<0.01），A组与C组比较差异无显著性（P>0.05）。PAI-1的表达水平B组低于A组和C组（P<0.01），A组与C组比较差异无显著性（P>0.05）。A、B、C三组总胆固醇（TC）水平分别为（23.51±10.58）、（14.27±3.51）和（1.36±0.33）mmol•L^-1，三组间两两比较差异有显著性（P<0.05）。A、B、C三组低密度脂蛋白（LDL）水平分别为（21.39±10.00）、（14.23±4.01）和（0.72±0.35）mmol•L^-1，三组间两两比较差异有显著性（P<0.05）。 甘油三酯（TG）水平三组间比较差异无显 著性（P>0.05）。结论：高脂饮食对兔动脉粥样硬化模型中NO和PAI-1表达水平无明显影响，阿托伐他汀干预使NO表达水平升高，使PAI-1表达水平降低，有利于抑制动脉粥样硬化的进程。

Expressions of nitric oxide and plasminogen activator inhibitor type-1 in rabbit models of atherosclerosis and interference effect of atorvastatin

Objective To investigate the expressions of nitric oxide (NO) and plasminogen activator inhibitor type-1(PAI-1)in rabbit models of atherosclerosis and the interference effect of atorvastatin. Methods Twenty four male Japanese rabbits were divided into 3 groups (8 in each group): high-fat diet group(group A),high-fat plus atorvastatin group (group B) and normal diet group(group C).The expressions of NO and PAI-1 were detected with ELISA assay.Plasma lipid was detected with automatic analysis divice. Results The espression of NO in group B was significantly higher than those in groups A and group C (P<0.01); there was no significant difference between groups A and C (P>0.05).The expression of PAI-1 in group B was significantly lower than those in groups A and C,there was no significant difference between groups A and group C (P>0.05).The levels of serum TC in groups A,B,and C were (23.51±10.58),(14.27±3.51)and(1.36±0.33)mmol·L-1, respectively; the levels of serum LDL in groups A,B,and C were (21.39±10.00),(14.23±4.01)and(0.72±0.35)mmol·L-1;there were significant differences between three groups (P<0.05),while TG had no significant difference between three groups (P>0.05).Conclusion High-fat diet has no significant effect on the expressions of NO and PAI-1 in atherosclerosis rabbits .Atorvastatin can increase the expression of NO and decrease the expression of PAI-1,and can inhibit the progression of atherosclerosis.