超抗原SEA对外周血T细胞的活化作用

目的 研究超抗原SEA对外周血T淋巴细胞的活化作用。方法 用SEA刺激人外周血T淋巴细胞，观察细胞的DNA合成、形态、IL-2的产生、细胞表型以及凋亡情况。结果 SEA对T淋巴细胞的促增殖作用的浓度为100mg/mL最强，在第2、3d达高峰；首次或多次刺激不改变T淋巴细胞的CD4^ CD8^ 的表型；首次刺激24h内未见明显凋亡；但再次刺激24h内即有明显凋亡；加入rhIL-22d凋亡现象消失。结论 超抗原SEA对T淋巴细胞的促增殖作用与其浓度、作用时间有关，并且SEA首次或多次刺激对CD4^ T淋巴细胞和CD8^ T淋巴细胞起同等的活化作用。

Study of activation of human T cells with superantigen SEA

ObjectiveTo investigate the activation of human T cells with superantigen SEA. MethodsT cells proliferation, IL 2 production, DNA synthesis, cell phenotype and apoptosis induced by the SEA in the first or restimulation were detected. Results It was shown that 100 ng/mL was the optimal activation concentration, IL 2 production arrived at top level in the third day, SEA activated CD4 + and CD8 +T with the same degree, T cells start apoptosis in response to SEA restimulation within 24 hours and apoptosis disappeared through addition of rhIL 2. Conclusions There were correlation between activation and SEA concentration or stimulation period; SEA activated both CD4 + and CD8 +T cells without change the cell phenotype.