| 缺氧预适应对缺氧复氧诱导内皮细胞-中性粒细胞黏附的影响 |
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| 引用本文： | 周四桂,雷小勇,廖端芳.缺氧预适应对缺氧复氧诱导内皮细胞-中性粒细胞黏附的影响[J].中国危重病急救医学,2003,15(3):159-162. |
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| 作者姓名： | 周四桂 雷小勇 廖端芳 |
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| 作者单位： | 南华大学药物药理研究所,湖南,衡阳,421001 |
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| 基金项目： | 国家"973"重点基础研究发展项目(G2000056905);国家自然科学基金资助项目(30171084);湖南省社会发展重大项目(01ssy200904). |
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| 摘 要： | 目的：观察缺氧预适应对缺氧／复氧后血管内皮细胞表面黏附分子的表达以及中性粒细胞－内皮细胞黏附的影响。方法：采用β－N－乙酰氨基己糖苷酶比色法检测黏附率;流式细胞术检测内皮细胞表面黏附分子E－选择素、细胞间黏附分子－1（ICAM－1）的表达;苔盼蓝摄取法检测细胞存活率;常规生化法检测乳酸脱氢酶活性。结果：血管内皮细胞经缺氧／复氧处理后,苔盼蓝摄取率,乳酸脱氢酶活性均明显增高,E－选择素、ICAM－1表达明显上调,其表面中性粒细胞的黏附增加,缺氧预适应显著抑制缺氧／复氧的上述作用。结论：缺氧预适应通过调节内皮细胞表面黏附分子的表达,抑制缺氧／复氧诱导的内皮细胞－中性粒细胞黏附。
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| 关 键 词： | 缺氧 复氧 细胞黏附分子 缺氧预适应 |
| 文章编号： | 1003-0603(2003)03-0159-04 |
| 修稿时间： | 2002年11月4日 |
Effects of hypoxic preconditioning on the adhesion of neutrophils to vascular endothelial cells induced by hypoxia/ reoxygenation |
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| ZHOU Sigui,LEI Xiaoyong,LIAO Duanfang.Effects of hypoxic preconditioning on the adhesion of neutrophils to vascular endothelial cells induced by hypoxia/ reoxygenation[J].Chinese Critical Care Medicine,2003,15(3):159-162. |
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| Authors: | ZHOU Sigui LEI Xiaoyong LIAO Duanfang |
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| Institution: | Institute of Pharmacy and Pharmacology, Nanhua University, Hengyang 421001, Hunan, China. |
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| Abstract: | Objective:To investigate whether hypoxic preconditioning (HPC) can inhibit neutrophils(PMN) adhesion to vascular endothelial cell(VEC).Methods:The adhesion of PMN to VEC was measured by endogenous enzyme hexosaminidase.The effect of HPC on the expression of endothelial cell(EC) adhesion molecules was determined with flow cytometry.Results:After stimulated with 1 hour of hypoxia(H) followed by 4 hours of reoxygenation(R),VEC showed an enhanced PMN adhensivity in association with an increased surface expression of Eselectin and intracellular adhesion molecules1(ICAM1).HPC suppressed the expression of Eselectin and ICAM1 with a subsequent inhibition of PMN adhesion to hypoxia/ reoxygenation (H/R) stimulated VEC.Conclusion:HPC inhibits PMN adhesion to VEC through regulating the expression of EC adhesion molecules. |
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| Keywords: | hypoxia reoxygenation endothelial cell adhesion molecule hypoxic precondition |
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