IL-24对CIK细胞体内杀伤肿瘤细胞活性的影响

目的 研究IL-24对细胞因子诱导的杀伤细胞(CIK)体内杀伤肿瘤细胞活性的影响。方法 (1)从健康人外周血中提取单个核细胞,第一天加入IFN-γ(1 000 IU/mL),第二天加入IL-1(100 IU/mL)、CD3mAb(50 ng/mL)、IL-2(300 IU/mL)诱导CIK细胞,另一组与IL-1、CD3mAb、IL-2同时加入IL-24(1 000 IU/mL);(2)建立HepG₂肿瘤细胞株荷瘤小鼠皮下移植瘤模型,分为五组,每三天分别测量肿瘤大小。结果 IL-24诱导CIK细胞治疗组荷瘤小鼠实体瘤体积小于其它组(P＜0.05)。结论 在CIK细胞诱导过程中加入IL-24能明显增强其体内的抗肿瘤活性,对临床上把CIK细胞用于生物治疗有一定的指导意义。

Effects of IL-24 on cytotoxic activity of cytokine-induced killer cells in vivo

Objective To study the effects of IL- 24 on eytotoxic activity of cytokineinduced killer cells in vivo. Methods （ 1 ） Mononuelear cells were extract from health aduh peripheral blood, one group added IFN -γ（ 1 000 IU/mL）at the first day and IL - 1 （ 100 IU/mL） ,CD3mAb（ 50 ng/mL） ,IL -2（ 300 IU/mL）at the second day. The other group added IL- 24 （ 1 000 IU/mL）at the same time with IL - 1, CD3mAb,IL -2;（ 2 ） Tumorbearing mice were divided into five groups,and tumor volumes were measured every three days. Results The solid tumor volumes of IL - 24 induced group are smaller than other groups （ P 〈 0.05 ）. Conclusion Added Ⅱ, - 24 during induced procedure can obviously enhance the cytotoxie activity of CIK cells in vivo.