Characterization of a novel HLA-Cw*02 variant, Cw*0208, in a Caucasian individual |
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Authors: | Canossi A Aureli A Piancatelli D Liberatore G Del Beato T Ozzella G Tessitore A Piazza A Papola F Casciani C U Adorno D |
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Institution: | CNR Institute, Organ Transplant and Immunocitology, 67100 L'Aquila, Italy. a.canossi@itoi.cnr.it |
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Abstract: | We describe an additional HLA-Cw*02 variant, HLA-Cw*0208, which has been identified in a renal transplant recipient of Caucasian origin (Italy). After performing preliminary serological typing, we analyzed exons 2 and 3 of the HLA-C locus polymorphism by cloning the amplified DNA and using a sequence-based typing method. The new allele differs from Cw*020202 by one nucleotide substitution at nucleotide 61 (G-->A) of exon 2, which translates to a difference of one amino acid at residue 21 (His-->Arg) of the HLA-C heavy chain. We propose that Cw*0208 was generated by a random point mutation in codon 21 from the Cw*020202 allele, or through gene conversion of Cw*020202 with another allele, probably the Cw*1205 and Cw*1602 alleles. |
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Keywords: | HLA-C HLA-Cw*0208 new alleles polymorphism sequence-based typing transplantation |
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