结肠腺瘤组织Ezrin蛋白和诱导型一氧化氮合酶表达及其生物学意义的探讨

目的探讨Ezrin蛋白、诱导型一氧化氮合酶(iNOS)在结肠腺瘤组织中的表达及其与腺瘤癌变的关系。方法应用免疫组织化学SP法检测60例结肠管状腺瘤、40例绒毛状及绒毛管腺瘤、30例腺瘤癌变中Ezrin蛋白、iNOS和Ki-67核抗原的表达及细胞定位。结果Ezrin蛋白、iNOS在结肠管状腺瘤[58.3％(35/60)、50.0％(30/60)]、绒毛状及绒毛管腺瘤[72.5％ (29/40)、70.0％(28/40)]、腺瘤癌变[96.7％(29/30)、93.3％(28/30)]中的表达分别依次上升，差异有统计学意义(Ezrin蛋白：x2= 11.600，P= 0.005;iNOS:X2= 8.451，P= 0.015)，Ezrin蛋白与iNOS表达有相关性(r=0.765，P＜0.01)；Ki-67核抗原增殖指数随Ezrin蛋白、iNOS表达上升而增加。结论Ezrin蛋白和iNOS的过表达可能与结肠腺瘤的增殖、恶性转化有关。

Expression of Ezrin and inducible nitric oxide synthase and their correlation in colonic adenoma

Objective To investigate the expression of Ezrin and inducible nitric oxide synthase (iNOS) and their correlation to malignant proliferation of colonic adenoma. Method Expressions of Ezrin,iNOS and nuclear Ki-67 antigen were detected by immunohistochemistry in tubular adenoma (60 cases),villous adenoma (40 cases) and malignant (30 cases ) pleomorphic adenoma. Results Expressions of Ezrin,iNOS in tubular adenoma [58.3％ (35/60), 50.0％ (30/60)], villous adenoma [72.5％ (29/40), 70.00(28/40)]and malignant pleomorphic adenoma [96.7％(29/30),93.3％(28/30)]were gradually increased ( X2 = 11.600,P = 0.005; X2 = 8.451, P = 0.015 ). There was a significant positive correlation between the expression of Ezrin and iNOS (r = 0.765, P ＜ 0.01 ). Nuclear Ki-67 antigen proliferation index was increased with the increasing of Ezrin and iNOS expressions. Conclusion Overexpression of Ezrin and iNOS may promote proliferation and malignant transformation of colonic adenoma.