Abstract: | Objective To improve the efficiency of anaerobic degradation of penicillin residues. Methods Different amounts of biochars were added in batch anaerobic fermentation at (35±2)℃. Results Adding biochars in the fermentation can make the biogas production increased by 42.8% over the control, the methane content peaked 3~5 days earlier than the control. The time to restore the pH to above 7.0 was significantly shortened. The VFA declined faster, and the ammonia nitrogen concentration below 2500mg/L. The VS removal rate increased by more than 20% when the organic loading were 2% VS and 3% VS. The biogas production increased by 175.2% compared with the control when the organic loading was 4% VS, but the system was still not operating normally. The fermentation system was acidified and did not start successfully when biochars were not added and the organic loading were 3% VS and 4% VS. Conclusion It is further illustrated that the addition of biochar can adjust the acid-base balance, alleviate the inhibition of ammonia nitrogen, increase the organic load of the system, and stabilize the |