鼻咽癌组织中微小RNA-328的表达及其对细胞侵袭和迁移的影响

目的 探讨鼻咽癌组织中微小RNA-328（miR-328）的表达及其对鼻咽癌CNE-2细胞侵袭和迁移的影响。方法 采用实时荧光定量PCR（QPCR）检测86例鼻咽癌组织和15例慢性鼻咽炎组织及鼻咽癌细胞系CNE-2、HK1和正常鼻咽上皮细胞NP69中的miR-328水平，分析miR-328水平与鼻咽癌临床病理特征（年龄、性别、临床分期、淋巴结转移、复发和生存状态）的关系；采用脂质体Lipofectamine 2000法向对数生长期CNE-2细胞转染miR-328模拟物（mimics）或阴性对照（NC），采用QPCR检测转染48 h后的miR-328水平以评价转染效率，采用Transwell迁移和侵袭实验检测转染后CNE-2细胞的穿膜细胞数目。结果 QPCR检测结果 显示，鼻咽癌细胞CNE-2、HK1中miR-328水平低于正常鼻咽上皮细胞株NP69（P＜0.05）；86例鼻咽癌组织的miR-328水平亦低于15例慢性鼻咽炎组织，差异有统计学意义（P＜0.05）。miR-328水平与鼻咽癌患者的复发状态（P=0.037）、临床分期（P=0.005）和生存状态（P=0.012）有关，但与年龄、性别和淋巴结转移无关（P＞0.05）。转染mimics 48 h后的细胞中miR-328水平大幅升高，与转染NC的细胞比较，差异有统计学意义（P＜0.05）。Transwell实验结果 显示，转染miR-328 mimics后，迁移和侵袭实验中CNE-2细胞的穿膜数目均降低，与转染NC的细胞比较，差异有统计学意义（P＜0.05）。结论 miR-328在鼻咽癌组织和细胞中低表达，且参与鼻咽癌的发生发展，上调其水平可抑制迁移侵袭过程，在鼻咽癌防治中有一定价值。

Expression of microRNA-328 in nasopharyngeal carcinoma tissues and its influence on cell invasion and migration

Objective To investigate the expression of microRNA-328 （miR-328） in nasopharyngeal carcinoma tissues and its effect on invasion and migration of CNE-2 cells. Methods The quantitative real-time PCR （QPCR） was used to detect the levels of miR-328 in 86 cases of nasopharyngeal carcinoma tissues and 15 cases of chronic nasopharyngitis as well as nasopharyngeal carcinoma cell lines CNE-2， HK1 and normal nasopharyngeal epithelial cell line NP69. The relationship between the levels of miR-328 and the clinicopathological features （age， sex， clinical stage， lymph node metastasis， recurrence and survival status） of nasopharyngeal carcinoma was analyzed. MiR-328 mimics or negative control （NC） were transfected into CNE-2 cells at logarithmic phase by liposome Lipofectamine 2000. The miR-328 level was detected by QPCR at 48 h after transfection to evaluate the transfection efficiency. The numbers of penetrating cells of CNE-2 cells were detected by Transwell migration and invasion assay. Results The Results of QPCR showed that the miR-328 levels in nasopharyngeal carcinoma cell lines CNE-2 and HK1 were lower than those in normal nasopharyngeal epithelial cell line NP69 （P＜0.05）， and the miR-328 levels in 86 cases of nasopharyngeal carcinoma tissues were lower than those in 15 cases of chronic nasopharyngitis （P＜0.05）. The miR-328 level was related to the recurrence （P=0.037）， clinical stage （P=0.005） and survival （P=0.012）， but not to age， sex and lymph node metastasis （P＞0.05）. After 48-h transfection， the miR-328 level in cells transfected with mimics was significantly higher than that in cells transfected with NC （P＜0.05）. Transwell assay showed that the numbers of CNE-2 cells transfected with mimics were significantly lower than those with NC （P＜0.05）. Conclusion miR-328 is lowly expressed in both nasopharyngeal carcinoma tissues and cells， and participates in the occurrence and development of nasopharyngeal carcinoma. Up-regulating its level can inhibit the migration and invasion process， which has a certain value in the prevention and treatment of nasopharyngeal carcinoma.