Abstract: | Objective To improve the quality of fine powder, the purification process of natamycin crude powder was optimized. Methods The orthogonal contrast test method was used to optimize the purification process conditions of natamycin, such as the dissolving agent of the crude powder, the concentration of the crude powder solution, the pH value of the crystal solution, the influence of washing and digging, and the type of digging and washing agent. Results The optimization of purification process of natamycin powder was successfully developed, namely, 75% methanol water solution as the dissolving agent, (2,5000±1000)u/mL solution concentration, 6.0~7.0 pH value of the crystal solution, and 75% acetone water solution as the agent to wash and dig the precipitate after crystallization. The purity of natamycin powder was 99.84%, the yield was 94.05%, and the impurity content was 0.2%. Conclusion After optimization, the quality of natamycin powder was greatly improved, and the process was stable and feasible, which could be used in the scale-up |