肝细胞癌组织微血管密度与血小板源性生长因子BmRNA表达的关系

目的初步探讨肝细胞癌组织中血小饭源性生长因子B（PDGPB）mRNA的表达与微血管密度（MVD）之间的关系。方法用RT-PCR法柃测51例肝细胞癌和20例正常肝组织PDGF-BmRNA。采用免疫组化（sABC）法检测MVD的表达。结果肝细胞癌组织PDGFBmRNA的表达量明显高于正常肝组织，两者比较有显著性差异（P〈0．01）。肝癌组织和正常肝组织的MVD值分别是29．43±4．818和14．56±1．96（P〈0．01）。结论PDGF-B作为一个血管内皮细胞生长调节因子，在肝癌的发生、发展过程中可能起着重要的促进生长和转移作用。

The research on relationship between expression of platelet-derived growth factor B and microvessel density in tissues of the hepatocellular carcinoma

Objective To investigate the relationship between platelet-derived growth factor B（PDGF-B）mRNA expression and mierovessel density（MVD）in liver tissues of bepatoeellular carcinoma （HCC）. Methods PDGF-B mRNAwas detected by RT-PCR in 51 eases of HCC and another 20 cases of normal liver tissues. At the same time,MVD expression was detected by immunobistocbemical staining. Results The expression of PDGF-B mRNA in HCC was significantly higher than that in normal liver tissues（P〈0.01）. The average MVD value was 29.43±4. 818 and 14.56±1.96,respectively in liver tissues of the two groups（ P 〈0.01）. The coefficient between PDGF-B and MVD was 0.331 （p= 0.018）. Conclusions PDGF-B,as a regulator of vascular endothelial cell growth factor,may play an important role in the development and metastasis of HCC.