口腔癌相关成纤维细胞对舌癌细胞株增殖活性的影响

目的本研究拟通过体外实验观察口腔癌相关成纤维细胞(carcinoma-associated fibroblasts,CAFs)对舌癌细胞增殖能力的影响。方法建立口腔CAFs与舌癌细胞株Tca8113的交互作用模型,对Tca8113进行形态学观察,并通过MTT法及流式细胞术,观测口腔CAFs对Tca8113增殖活性及细胞周期的影响。结果CAFs和Tca8113直接混合培养组的Tca8113细胞形态及生长方式发生变化;与正常成纤维细胞(normal fibroblasts,NFs)相比,CAFs增强了Tca8113的增殖活性(P<0.05),并提高了处于S期和G2期的癌细胞的比例(48.1%vs40.0%)。结论口腔CAFs可在体外促进舌癌细胞株Tca8113的增殖,提示其在口腔鳞癌发展中起重要作用。

The Study on Oral Carcinoma-associated Fibroblast Promoting the Proliferation of Lingual Carcinoma Cell Line

Objective The aim of this study was in vitro to investigate the effects of oral carcinoma-associated fibroblasts(CAFs) on the proliferation of lingual carcinoma cells.Methods The interaction model between primary oral CAFs and a lingual carcinoma cell line Tca8113 was established for this study project.The effects of CAFs on the viability and cell cycle of Tca8113 were investigated through morphological observation,MTT assay and flow cytometry.Results After oral CAFs interacted with Tca8113 directly,Tca8113 showed the cellular shape changes in morphology;compared with normal fibroblasts(NFs),CAFs enhanced the viability of Tca8113(P<0.05),and increased the percentage of carcinoma cells in S phase and G2 phase(48.1% vs 40.0%).Conclusion Oral CAFs can promote the proliferation of the lingual carcinoma cell line Tca8113 in vitro,and may play a key role in the progression of oral squamous cell carcinoma(OSCC).