Improvement of quality control for steroid receptor measurements: Analysis of distributions in more than 40000 primary breast cancers |
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Authors: | Sylvie Romain Frédérique Spyratos Jacques Goussard Jean-Louis Formento Henri Magdelénat |
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Affiliation: | (1) Laboratoire d'Oncologie Biologique — Biopathologie Tissulaire, APM, Faculté de Médecine Nord, Bd Pierre Dramard, 13916 Marseille Cedex 20, France;(2) Département de Biologie, Centre René Huguenin, St Cloud, France;(3) Laboratoire d'Oncobiologie, Centre François Baclesse, Caen, France;(4) Laboratoire d'Oncopharmacologie, Centre Antoine Lacassagne, Nice, France;(5) Laboratoire de Transfert, Institut Curie, Paris, France |
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Abstract: | Summary All French laboratories that routinely assay estradiol (ER) and progesterone (PR) receptors participate in the European EORTC quality control program based on twice-yearly analysis of 5 cytosolic preparations. This system has considerably reduced inter-laboratory variations, but does not cover all aspects of these assays. Analysis of receptor value distributions is also crucial to ensure that receptor measurements remain stable with time, independently of the laboratory and assay method. This study involved 83907 receptor assays carried out in the last 17 years by 17 laboratories belonging to the French Study Group on Tissue and Molecular Biopathology. The assays were based on radioligand binding (RLA) or immunoenzymology (EIA). For each laboratory, the medians and positivity rates were analysed according to two totally objective criteria, the patient's age and the year of assay, and according to histological grade and histological type of the tumor in order to verify the correlations classically described. Age-related distributions varied little between laboratories, compared with data published by 7 European EORTC laboratories [1]. The results remained relatively stable with time in the RLA method for ER and PR, and in the EIA method for PR. Median ER-EIA data showed a marked increase between 1987 and 1989, mainly due to changes in the quality of Abbott reagents during this period. Otherwise, this analysis confirms previous pathophysiological observations.List of participants: J.P. Basuyau, P. Brunelle, Centre Henry Becquerel, Rouen; G. Besse, Centre Jean Perrin, Clermond Ferrand; E.M. Chambaz, M. Chedin, S. Bottari, CHRU, Grenoble; A. Daver, Centre Paul-Papin, Angers; J.C. Delarue, Institut Gustave Roussy, Villejuif; J. Goussard, Centre François Baclesse, Caen; J. Grenier, CRLCC Val d'Aurelle, Montpellier; J.C. Jardillier, A. Rallet, Institut Jean Godinet, Reims; P. Jolliet, Centre Intercommunal, Creteil; M. Lanson, P. Bougnoux, CHU Bretonneau, Tours; H. Magdelénat, Institut Curie, Paris; P.M. Martin, S. Romain, Faculté de Médecine Nord, Marseille; G. Milano, J.L. Formento, Centre Antoine Lacassagne, Nice; J.P. Peyrat, Centre Oscar Lambret, Lille; V. Quillen-Pouvreau, Centre E. Marquis, Rennes; G. Ricolleau, Centre René Gauducheau, Saint-Herblain; H. Rochefort, J.P. Brouillet, Hôpital A. de Villeneuve, Montpellier; G. Soula, P. Courrière, Centre Claudius Regaud, Toulouse; F. Spyratos, Centre René Huguenin, Saint-Cloud; J. Wafflart, Fondation Bergonié, Bordeaux. |
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Keywords: | breast cancer estrogen receptor inter-laboratory variation progesterone receptor |
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