p16基因表达与胃癌组织类型的关系

目的：探讨ｐ１６基因的表达与人胃癌组织类型的关系。方法：选取２４例病理确诊的胃癌组织及相应正常胃粘膜组织，用蛋白质免疫印迹（Ｗｅｓｔｅｒｎ ｂｌｏｔ）技术检测ｐ１６基因在两种组织中的表达。结果：２４例胃癌组织中，６例无ｐ１６基因表达的癌组织为低分化腺癌或印戒细胞癌；２例ｐ１６基因表达可疑；分化较高的５例ｐ１６基因表达增多；其们１１例ｐ１６基因的表达同相应正常组织无区别。结论：ｐ１６基因在胃癌组织中

Relationship between the Expression of p16 Gene and the Phenotypes of Human Gastric Carcinomas

Background/Aims: To study the relationship between the expression of p16 gene and the phenotypes of human gastric carcinomas. Methods: 24 human gastric carcinoma specimens and the adjacent normal gastric mucosal tissues were determined by molecular pathologic methods. The expression of p16 gene was studied by Western blot assay. Results: Deleted p16 gene expression was found in 6 poorly-differentiated adenocarcinomas or signet ring cell carcinomas among the 24 human gastric carcinomas, in two, the p16 gene expression was suspicious, and in 5 well-differentiated carcinomas, it was over-expressed, while in the rest of 11 cases, there were no differences between cancer tissue and the normal gastric mucosal tissue. Conclusions: The expression of p16 gene in human gastric carcinomas may be either decreased or increased, and in many cases, the gastric carcinomatous tissue showed no difference as compared with the adjacent normal gastric mucosal tissue; the cases of decreased expression of p16 gene always belong to the poorly-differentiated ones or the signet ring cell carcinomas,which might be related to their poor prognosis.