| 抗人C1q单克隆抗体检测方法——C1q-ELISA的建立 |
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| 引用本文： | 谢佩蓉,冯庆和,赵菲莎,王剑平,朱锡华. 抗人C1q单克隆抗体检测方法——C1q-ELISA的建立[J]. 第三军医大学学报, 1986, 0(1) |
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| 作者姓名： | 谢佩蓉 冯庆和 赵菲莎 王剑平 朱锡华 |
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| 作者单位： | 第三军医大学免疫学研究室(谢佩蓉，冯庆和，赵菲莎，王剑平)，第三军医大学免疫学研究室(朱锡华) |
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| 摘 要： | 为检测抗人C1q单克隆抗体,我们建立了固相C1q-ELISA。经取代试验、阻断试验及交叉试验证明本法具有特异性;同时用C1q-ELISA及双向免疫扩散试验检测15份鼠抗人C1q血清抗体滴度,表明C1q-ELISA法较双向免疫扩散法高20156倍,用6份血清试验批内及批间的变异系数范围,分别为1.8～6.5％及6.2～16.5％,说明有较好的重现性。因而,本法适用于杂交瘤细胞上清中抗人C1q单克隆抗体的检测。
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| 关 键 词： | C1q 单克隆抗体 酶联 免疫吸附试验 |
Establishment of Clq-ELISA for Detection of Antihuman Clq Monoclonal Antibodies |
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| Xie Peirong,et al. Establishment of Clq-ELISA for Detection of Antihuman Clq Monoclonal Antibodies[J]. Acta Academiae Medicinae Militaris Tertiae, 1986, 0(1) |
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| Authors: | Xie Peirong et al |
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| Abstract: | The technique of Clq-ELISA has been established in this laboratory for the detection of antihuman Clq monoclonal antibodies(McAb). This technique, as verified by displacement test, blocking test, and cross titration test, possesses high specificity. It is more sensitive than double immunodiffusion as verified by the tests of 15 specimens of mouse antihuman Clq serum and has better reproducibility. It is concluded that this technique is very useful for the detection of anti-Clq McAb in the supernatant of hybridoma culture. |
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| Keywords: | C1q Monoclonal antibodies ELISA |
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