原发性胃腺癌P53基因第7、8外显子突变研究

目的：探讨连云港地区原发性胃腺癌P53基因变异发性情况，方法：应用聚合酶链反应单链构象多态分析法（PCR－SSCP）对20例原发性胃腺癌的P53基因第7、8外显子突变进行了检测。结果：共发现突变5例（25％），其中第7外显子突变4例，第8外显子突变1例，在20例原发性胃腺癌组织分型中，高分化和中分化各3例，均未见P53基因第7、8外显子突变，低分化13例，发现P53基因第7外显子突变3例，第8外显子突变1例；低分一未分化1例，亦发现第7外显子突变。结论：P53基因突变与原发性胃腺癌发生有关；对原发性胃腺癌的治疗有指导意义。

Study of the 7th and 8th Exon Mutation of 53 Cene in Primary Stomach Cancer Tissue

Objective:To study the 7th and the 8th exon mutation of P53 gene in primary gastric adenocarcinoma. Methods:PCR-SSCP was taken to examine the 7th and 8th exon mutation of P53 gene in 20 cases of primary gastric adenocarcinoma.Results:5 cases of the 7th and 8th exon mutation of P53 gene were found(25%),3 cases of the 7th exon mutation and one case of the 8th exon mutation.Among 20 cases of primary gastric adenocarcinoma,there were 3 cases of well-differentiated ones and 3 cases of middle-differentiated ones in which the 7th and 8th exon mutation of P53 gene were not found;13 cases of poorly-differentiated ones in which 3 cases of the 7th exon mutation and one case of the 8th exon mutation of P53 gene were found;one case of poorly-undifferentiated ptimary gastric andenocarcinoma which showed the 7th exon mutation of P53 gene.Conclusion:The mutation of P53 gene is correlated to primary gastric andenocarcinoma,which is of instructive importance to the treatment of primary gastric andenocarcinoma.