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mRNA expression of T-helper 1, T-helper 2 cytokines in autoimmune hepatitis in childhood |
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| Authors: | Hisashi Kawashima Naoki Kato Hiroaki Ioi Shigeo Nishimata Chiako Watanabe Yasuyo Kashiwagi Kouji Takekuma Akinori Hoshika Leszek Szenborn Kacprzak Bergman |
| Institution: | Department of Pediatrics, Tokyo Medical University, Tokyo, Japan; and Clinical Infectious Diseases of Children, Institute of Pediatrics, Medical Academy, Wroclaw, Poland |
| Abstract: | Background: In the pathology of autoimmune hepatitis the immunity mechanism of T-helper 1 (Th1) and Th2 cells was recently evaluated. The purpose of the present study was to measure the mRNA levels in peripheral mononuclear cells and serum cytokines obtained from children with autoimmune hepatitis for a better understanding of the mechanism. Methods: Twenty-five patients with autoimmune hepatitis and seven controls were enrolled. mRNA levels in peripheral mononuclear cells and serum cytokines were measured using real-time polymerase chain reaction and immunoassay. Results: Serum interferon-γ (IFN-γ) and interleukin-4 (IL-4) were rarely detected. In contrast the IFN-γ/β-actin mRNA levels were high. Conclusion: Autoimmune hepatitis is a Th1-predominant state, therefore immune modulation therapies that target the control of Th1 cytokines should be used. |
| Keywords: | autoimmune hepatitis childhood mRNA Th1 Th2 |