| 5α-△9(11)-16α-甲基-3β,17α,21-三羟基-孕甾烯-3β,21-双醋酸酯-20酮的微生物转化 |
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| 引用本文： | 张丽青,王敬一.5α-△9(11)-16α-甲基-3β,17α,21-三羟基-孕甾烯-3β,21-双醋酸酯-20酮的微生物转化[J].药学学报,1986,21(9):674-679. |
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| 作者姓名： | 张丽青 王敬一 |
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| 作者单位： | 中国科学院上海有机化学研究所,*中国科学院上海生物工程实验基地 |
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| 摘 要： | 简单节杆菌A1及耻垢杆菌MS1两种菌混合培养转化地塞米松中间体5α-△9(11)-16α-甲基-3μ,17μ,21-三羟基-孕甾烯-3μ,21-双醋酸酯-20酮(Ⅲ)可得到16α-甲基-△1.4.0(11)-孕甾烯-17α,21-双羟基-3,20双酮(Ⅳ)(65%收率)及少量的16α-甲基-△1.4孕甾烯-9α,11α-环氧-17α,21双羟基-3,20双酮(Ⅴ)。
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| 关 键 词： | 微生物转化 简单节杆菌 耻垢杆菌 混合培养 地塞米松 |
| 收稿时间： | 1985-11-13 |
Microbial transformation of 16 alpha-methyl-5 alpha-delta 9(11)- pregnene-3 beta, 17 alpha, 21-triol-20-one-3 beta, 21-diacetate |
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| ZHANG Li-Oing and WANG Jing-Yi.Microbial transformation of 16 alpha-methyl-5 alpha-delta 9(11)- pregnene-3 beta, 17 alpha, 21-triol-20-one-3 beta, 21-diacetate[J].Acta Pharmaceutica Sinica,1986,21(9):674-679. |
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| Authors: | ZHANG Li-Oing and WANG Jing-Yi |
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| Abstract: | Incubation of 16α-Methyl-5α-△9(11)-Pregnene-3β, 17α, 21-triol-20-one 3β, 21-diacetate (Ⅲ)with a mixed culture of Mycobacterium smegmatis (MS1) and Arthrobacter simplex (A1) gave 16α-methyl-1,4,9(11) Pregnatriene-17α, 21-dihydroxy-3,20-dione (Ⅳ) (65% yield) and a small amount of 16α-methyl-△1,4-pregnadiene-9α, 11α-epoxy-17α, 21-diol-3, 20-dione (Ⅴ). At 0.5% substrate concentration, Ni ion was shown to be needed to inhibit the production of the byproduct compound(Ⅴ) and increase the yield of compound(Ⅳ). |
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| Keywords: | Arthrobacter simplex Mycbacterium smegematis Mixed culture Dexamethasone Microbial transformation |
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