| 特异性阻断Shh信号通路对胃癌细胞系SGC-7901增殖和凋亡的影响 |
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| 引用本文： | 李玖池,苏继荣,闫慧明,张志军,卢永清,李文龙,武伟. 特异性阻断Shh信号通路对胃癌细胞系SGC-7901增殖和凋亡的影响[J]. 临床医药实践, 2008, 17(3): 166-168 |
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| 作者姓名： | 李玖池 苏继荣 闫慧明 张志军 卢永清 李文龙 武伟 |
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| 作者单位： | 山西医科大学第二临床医学院,山西,太原,030001 |
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| 摘 要： | 目的:研究Sonic Hedgehog(Shh)信号通路对胃癌细胞系SGC-7901增殖和凋亡的影响。方法:用四唑蓝(MTT)比色试验检测Shh信号通路特异性抑制剂cyclopamine对胃癌细胞系SGC-7901增殖的抑制作用;流式细胞术检测细胞增殖指数(PI)和凋亡指数(AI)。结果:Cyclopamine对SGC-7901细胞株增殖的抑制作用呈剂量和时间依赖性;Cyclopamine作用后使胃癌细胞周期阻滞在G0/G1期,细胞凋亡增加。结论:Shh信号分子对胃癌细胞的增殖起维持作用;通过特异性阻断该信号通路,可以抑制胃癌细胞增殖,并促进细胞凋亡。
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| 关 键 词： | 信号通路 胃癌细胞 流式细胞术 |
| 文章编号： | 1671-8631(2008)03-0166-03 |
| 修稿时间： | 2007-12-17 |
Effect of sonic hedgdhog signal pathway blocking on the proliferation and apoptosis of gastric cancer cell line SGE-7901 |
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| LI Jiu-chi,SU Ji-rong,YAN Hui-ming,ZHANG Zhi-jun,LU Yong-qing,LI Wen-long,WU Wei. Effect of sonic hedgdhog signal pathway blocking on the proliferation and apoptosis of gastric cancer cell line SGE-7901[J]. Proceeding of Clinical Medicine, 2008, 17(3): 166-168 |
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| Authors: | LI Jiu-chi SU Ji-rong YAN Hui-ming ZHANG Zhi-jun LU Yong-qing LI Wen-long WU Wei |
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| Abstract: | Objective:To investigate the effect of sonic hedgehog signal pathway,on the proliferation and apoptosis of gastric cancer cell line SGC-7901.Methods:Use the MTT assay to examine the antiproliferation effects of cyclopamine on the gastric cancer cell line SGC-7901.Use the flow cytometry(FCM) to examine the effects of cyclopamine on the proliferation index(PI) and apoptosis index(AI) of SGC-7901 cells.Results:The antiproliferative effects of cyclopamine produced a kind of dependence on dose and time.It could induce apoptosis and block cell cycle at G0/G1 phase in treating SUC-7901 cells with cyclopamine.Conclusion:Shh signal pathway is essential for maintaining gastric cancer cell proliferation.Blocking Shh pathway can inhibit the growth of gastric cancer cell and promote the apoptosis. |
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| Keywords: | sonic hedgehog gastric cancer cell flow cytometry |
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