新生儿脐血体外感染呼吸道合胞病毒后IL-6基因表达的变化

目的　研究呼吸道合胞病毒（ Ｒ Ｓ Ｖ） 对新生儿脐带血白细胞介素 ６（ Ｉ Ｌ ６） 基因表达的影响。方法　体外培养新生儿脐带血单个核细胞,用 Ｒ Ｓ Ｖ 和灭活 Ｒ Ｓ Ｖ 体外感染培养的单个核细胞,用反转录聚合酶链反应技术（ Ｒ Ｔ Ｐ Ｃ Ｒ） 检测感染后不同时间 Ｉ Ｌ ６ 基因表达的变化。用酶切鉴定其产物特异性。以正常成人外周血为对照。结果　 Ｒ Ｓ Ｖ 感染后６ 小时脐带血和成人血单个核细胞均有 Ｉ Ｌ ６ ｍ Ｒ Ｎ Ａ 的高表达,但１２ ～２４ 小时后脐带血及灭活 Ｒ Ｓ Ｖ 感染的脐带血无 Ｉ Ｌ ６ ｍ Ｒ Ｎ Ａ 表达。结论　新生儿脐带血和成人外周血在 Ｒ Ｓ Ｖ 感染后都具备产生免疫调节因子的能力,非复制的灭活病毒对新生儿没有保护作用。

Expresssion of IL 6 in cord bloods of neonate infected with espiratory syntycial virus(RSV)

Objective To study the effect of RSV on the gene expression of IL 6 in neonate.Methods mononuclear cells of cord bloods in neonate in vitro were cultured and infected with active RSV and inactivated RSV.Reverse transcription polymerase chain reaction(RT PCR) was used to detect the gene expression of IL 6 in different time after infection.Restriction enzymes was used to identify the specificity of RT PCR products.Peipheral bloods from normal adults were as controls.Results I 6 mRNA was highly expressed in mononuclear cells of cord blood and adult blood at 6 hours after infection.No IL 6 expression was detected in cord blood during the period of 12 24 hours of infection and inactivated infection.Conclusion Either neonate or adult has ability to reproduce immune cytokines and nonreplicating RSV has no protecting effect on neonate.