鼠腓总神经端侧缝合后脊髓内HRP阳性标记神经细胞出现的时间和数量

目的用辣根过氧化物酶（ｈｏｒｓｅｒａｄｉｓｈｐｅｒｏｘｉｄａｓｅ，ＨＲＰ）追踪法比较神经端侧缝合与对端缝合后脊髓内神经细胞出现的时间和数量。方法Ｗｉｓｔａｒ大鼠３６只，切断其右侧腓总神经后，按手术方法随机分为３组，每组１２只。（Ａ）端侧吻合组：腓神经远断端与胫神经侧方窗口缝合。（Ｂ）对端缝合组：腓总神经近、远端作对端缝合。（Ｃ）对照组：腓总神经不作缝合。术后２、４、６、８、１０及１２周，３组各取２只鼠，自腓总神经最远端注射０．１μｌＨＲＰ（３０％），７２小时后取出大鼠Ｔ１２～Ｌ６脊髓节段和相应的后根神经节，作组织学观察。结果Ａ组：术后１２周时，同侧Ｌ２～５后根神经节，脊髓后角、前角出现典型的ＨＲＰ阳性标记细胞，比Ｂ组慢６周。细胞数量比Ｂ组少４５．７％（ｔ＝４．９２，Ｐ＜０．０１）。Ｃ组则一直未见ＨＲＰ阳性标记细胞。结论神经端侧缝合后可以再生，但其速度和阳性细胞数量均较对端缝合差。

Study of the effect of end to side coaptation on rat common peroneal nerve by HRP tracing method

Objective To compare the effect of end to side coaptation with that of end to end coaptation on peripheral nerve regeneration in rats,by means of horseradish peroxidase(HRP) tracing. Methods 36 Wistar rats were randomly divided into three groups after the right common peroneal nerve was transected. In group A,the distal end of the right common peroneal nerve was sutured to the side of the tibial nerve through a perineurial window. In group B, the distal end of the right common peroneal nerve was sutured to the proximal end.No neurorrhaphy of the common peroneal nerve was performed in group C (control group). 0.1 l of 30 % HRP was injected into the trunk of the right common peroneal nerve distal to the suture site. 72 hours later, the rats were sacrificed to harvest the spinal cord segment and the dorsal root ganglion of T12 to L6 segments for histological study. Results Positive HRP marked cells were found in the spinal ganglion, the posterior horn and the anterior horn of the spinal cord on the twelfth postoperative week in group A. Compared with those in group B, postive HRP marked cells in group A appeared 6 weeks later, and the number was 45.7 % fewer. No positive HRP marked cells was found in group C. ConclusionsPeripheral nerve regeneration could be achieved after end to side coaptation, but was inferior to that obtained after end to end coaptation.