谷胱甘肽和牛磺酸对汞急性肾毒性影响的实验研究

目的 探讨预先投予GSH和牛磺酸对汞急性肾毒性的影响。方法 将32只Wistar大鼠随机分为对照、HgCl2染毒、GSH HgCl2和牛磺酸 HgCl2 4组。对照组皮下注射0．9％的生理盐水，汞染毒组皮下注射2．5mg／kg的HgCl2溶液，注射容量为5ml／kg。GSH和牛磺酸组于注射相同剂量Hg口2前2h分别腹腔注射3mmoL/kg的GSH溶液和4mmol／kg的牛磺酸溶液。测定尿N-Z乙酰-β-D氨基葡萄糖苷酶(NAG)、碱性磷酸酶(ALP)、乳酸脱氢酶(LDH)活性，尿蛋白和尿肌酐含量，血清尿素氮(BUN)以及尿汞和肾汞含量。结果 与对照组比较，HgCl2染毒组尿NAG、ALP、LDH活性显著升高，尿蛋白、BUN、尿汞、肾汞含量明显增加。预先投予GSH和牛磺酸后，可使汞染毒大鼠尿NAG和ALP活性、尿蛋白和BUN含量均较HgCl2染毒组显著减低；GSH组尿汞显著低于单纯染汞组，而牛磺酸组尿汞、肾汞与汞染毒组比较差异则无显著性。结论 GSH和牛磺酸对汞致急性肾损伤有一定的保护作用。

Experimental study on the effect of glutathione and taurine on acute nephrotoxicity caused by mercury

Objective To study the effects of glutathione(GSH)and taurine on acute nephrotoxicity caused by mercury(Hg).Method Thirty-two Wistar rats were randomly divided into four groups.Control rats were given subcutaneous injections with 0.9% saline.Rats in mercuric chloride(HgCl 2)group were subcutaneously injected with 2.5 mg/kg HgCl 2.Other two rat groups were pretreated with 3 mmol/kg GSH and 4 mmol/kg taurine,respectively and two hours later injected subcutaneously with 2.5 mg/kg HgCl 2.Urine activities of NAG,ALP,LDH,urine protein and mercury levels were determined.Serum level of BUN and content of mercury in the renal cortex of rats were measured.Result As compared with the controls,urine activities of NAG,ALP,LDH,urine protein and mercury levels,serum level of BUN and content of mercury in the renal cortex in the rats with 2.5 mg/kg HgCl 2 all increased significantly.Urine activities of NAG and ALP,urine level of protein and serum level of BUN decreased significantly in the rats pretreated with GSH and taurine,as compared with those injected with mercury alone.Urine level of mercury in the GSH group was significantly lower than that in the mercury group.As compared with the mercury group,urine level of mercury in the rats with taurine and content of mercury in the renal cortex in the rats with GSH and taurine all had no significant difference.Conclusion Pretreated with GSH and taurine had certain protection against acute nephrotoxicity caused by mercury.