两种方法检查新生儿溶血病的比较分析

目的用两种方法检测新生儿母婴血型不合溶血病,分析二者之间有无差异.方法主要检测方法是患儿红细胞直接抗人-IgG试验、血清游离抗体检测及患儿红细胞放散液鉴定3项试验,用传统的抗人-IgG试验与戴安娜卡式系统比较.结果用抗人-IgG法检测210例,阳性51例,占24.29%.用卡式检测375例,阳性129例,占31.7%.结论采用两种方法,对3项试验中游离抗体鉴定与放散液鉴定的结果比较,卡式阳性检出率明显高于传统的抗人-IgG法,操作简单,结果稳定且易保存,灵敏度高,能检测到十分微弱的抗原抗体反应和不完全抗体的存在.样本需用量少,有利于新生儿及少血样本的检测.

Comparative analysis of two methods for examination of newborn hemolysis

Objective To explore and compare the difference between the two methods for the determination of newborn hemolysis due to mismatched mother and newborn blood type.Methods The major detecting methods included the test of sick newborn erythrocytic direct anti-human-IgG,detection of serum free antibody and the identification of sick newborn erythrocyte dispersion.The traditional anti-human-IgG test was compared with Dianna casette system.Results The positive rate was 24.29%(51 positive out of 210 cases) by anti-human-IgG method and 31.7%(129 positive out of 375 cases) by the casette method.Conclusion The positive rate by casette method is obviously higher than that by traditional anti-human-IgG method.And the casette method is simple with stable results and high sentivitity.And smaller quantity of sampling is beneficial for the examination of newborn test.