口虾蛄提取物对人鼻咽癌细胞迁移和体外血管生成拟态的抑制作用

 目的：研究口虾蛄提取物（extract of Oratosquilla，EOS）对人低分化鼻咽癌细胞株CNE-2的迁移及体外血管生成拟态的影响。方法：用不同浓度（0 mg/L 、125 mg/L、250 mg/L、500 mg/L）EOS处理CNE-2细胞24 h后，创伤修复实验检测细胞迁移能力；通过Matrigel三维细胞培养观察CNE-2细胞形成类血管网状结构的能力及其特点；体外管道形成抑制实验检测不同浓度EOS对CNE-2细胞管道形成能力的影响；Western blotting法检测不同浓度EOS对CNE-2细胞fascin 1和血管内皮生长因子（VEGF）蛋白表达的影响。结果：EOS可以显著降低CNE-2细胞的迁移能力，与对照组比较差异有统计学意义（P＜0.01）；CNE-2细胞在Matrigel上培养能形成类似血管的网状样结构；EOS能以剂量依赖方式抑制CNE-2细胞体外管道形成的数量（P＜0.01）；EOS能抑制CNE-2细胞中fascin 1和VEGF蛋白的表达（P＜0.01），且其管状结构数量与2种蛋白变化趋势呈正相关（P＜0.05）。结论：CNE-2细胞具有血管生成拟态的能力；EOS能够抑制CNE-2细胞的迁移和体外血管生成拟态的能力，其机制可能与下调fascin 1和VEGF蛋白的表达有关。

Inhibitory effect of extract of Oratosquilla on migration and vasculogenic mimicry in human nasopharyngeal carcinoma cell line in vitro

AIM: To study the inhibitory effect of the extract of Oratosquilla (EOS) on the migration and vasculogenic mimicry in human poorly differentiated nasopharyngeal carcinoma cell line CNE-2.METHODS: CNE-2 cells were cultured in the medium with different concentrations of EOS (0 mg/L, 125 mg/L, 250 mg/L and 500 mg/L). The migration of CNE-2 cells and the formation of tube-like structures (TLSs) by CNE-2 cells were determined with wound healing assay and in vitro anti-angiogenesis test, respectively. The formation of TLSs by CNE-2 cells and their structural characteristics were observed by anti-angiogenesis test on the Matrigel. The protein expression of fascin 1 and vascular endothelial growth factor (VEGF) was detected by Western blotting.RESULTS:  Compared with control group, EOS significantly decreased the migration velocity of CNE-2 cells in a dose-dependent manner. CNE-2 cells formed TLSs on the Matrigel, and the formation of TLSs by CNE-2 cells was inhibited by EOS in a dose-dependent manner. The expression of fascin 1 and VEGF in CNE-2 cells was also decreased after treatment with EOS. A positive correlation between the expression of fascin 1/VEGF and the formation of TLSs by CNE-2 cells   was observed.CONCLUSION: CNE-2 cells form TLSs on the Matrigel, and EOS inhibits the migration and vasculogenic mimicry of CNE-2 cells, which are related with down-regulating the expression of fascin 1 and VEGF in CNE-2 cells.