| 周围神经43kD蛋白单克隆抗体的制备 |
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| 引用本文： | 何江虹,王东,张沛云,丁斐,顾晓松. 周围神经43kD蛋白单克隆抗体的制备[J]. 南通医学院学报, 2001, 21(4): 337-338 |
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| 作者姓名： | 何江虹 王东 张沛云 丁斐 顾晓松 |
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| 作者单位： | 南通医学院 |
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| 基金项目： | 国家自然科学基金资助 ( 30 0 70 2 5 5 )，江苏省科技厅社会发展基金资助 ( BJ2 0 0 32 2 ) |
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| 摘 要： | 目的：制备周围神经43kD蛋白单克隆抗体。方法：通过SDS－聚丙烯酰胺凝胶系统，从周围神经组织中分离回收43kD蛋白作为抗原，免疫BALB／C小鼠，通过杂交瘤技术，点膜印迹法检测，获得相应的分泌单克隆抗体的杂交瘤细胞株，并以Western blot方法检测抗体的特异性。结果：阳性克隆可持续稳定分泌抗43kD蛋白抗体，Western Blot显示在43kD处出现特异的阳性反应条带。结论：建立了分泌抗43kD蛋白单克隆抗体的杂交瘤细胞株，获得了43kD蛋白单克隆抗体。
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| 关 键 词： | 周围神经 43kD蛋白 单克隆抗体 制备 |
| 文章编号： | 1000-2057(2001)04-0337-02 |
| 修稿时间： | 2001-06-30 |
THE PREPARATION OF MONOCLONAL ANTIBODY AGAINST 43kD PROTEIN OF PERIPHERAL NERVE |
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| HE Jianghong,WANG Dong,ZHANG Peiyun,et al. THE PREPARATION OF MONOCLONAL ANTIBODY AGAINST 43kD PROTEIN OF PERIPHERAL NERVE[J]. ACTA Academiae Medicinae Nantong, 2001, 21(4): 337-338 |
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| Authors: | HE Jianghong WANG Dong ZHANG Peiyun et al |
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| Abstract: | Objective: To prepare the monoclonal antibody against 43kD protein of peripheral nerve.Methods:The 43kD protein,as antigen,was isolated from peripheral nerve by SDS-PAGE . The monoclonal antibody had been prepared through immunizing the BALB/C mouse with 43kD protein by assay of dot blot. And Western Blot had been employed to demonstrate the specificity of the monoclonal antibody.Result: Special positive band was found on 43kD by Western Blot. Conclusion: The monoclonal antibody against 43kD protein has been developed. |
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| Keywords: | peripheral nerve 43kD protein monoclonal antibody |
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