阻断FAK表达对涎腺腺样囊性癌细胞SACC—LM的侵袭行为的影响

目的：研究探讨阻断黏着斑激酶（focal adhesion kinase，FAK）表达对涎腺腺样囊性癌肺转移亚系细胞（salivary adenoid cystic carcinoma-Lung metastasis，SACC—LM）侵袭行为的影响及相关机制。方法：应用酪氨酸蛋白激酶抑制剂Genistein处理体外培养的SACC-LM细胞，应用transwell小室观察Genistein对SACC—LM细胞侵袭的影响，并应用RT-PCR法检测FAK和细胞外信号调节激酶（extracellular signal—regulated kinase，ERK）mRNA的表达。所得实验数据采用SPSS10．0统计软件t检验进行分组分析。结果：Genistein使SACC-LM细胞侵袭能力减弱；检测到随着Genistein抑制剂浓度的增高和作用时间的延长，FAK和ERK mRNA的表达水平均降低。结论：阻断FAK表达导致SACC-LM细胞侵袭能力减弱，原因可能是FAK和ERK表达水平的改变。

The effects of blocked the expressions of focal adhesion kinase and extracellular signal-regulated kinase on the invasion behavior of salivary adenoid cystic carcinoma cell line SACC-LM

Objective： To investigate the invasion effects of blocking the expressions of FAK and ERK in the behavior of salivary adenoid cystic carcinoma-lung metastasis cell SACC-LM. Methods： SACC-LM cells cultured in vitro were treated with the tyrosine protein kinase of genistein, and observed the effects of the invasions of SACC-LM cells by genistein, the expressions of FAK and ERK mRNA were detected with RT-PCR, t test was performed using SPSS 10.0 software. Results： The invasion ability of SACC-LM cells dropped when treated with genistein, Blocked expressions of FAK could dropped the invasion ability of SACC-LM cells. The level of FAK and ERK mRNA changed obviously after blockade. Conclusion： Blocked expressions of FAK could change the invasion ability of SACC-LM cells, which might be related with the expression changes of FAK and ERK mRNA.