| LM23基因沉默引起大鼠睾丸生精细胞凋亡 |
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| 引用本文： | 石心泉,贾孟春,刘美玲. LM23基因沉默引起大鼠睾丸生精细胞凋亡[J]. 生殖医学杂志, 2010, 19(4): 337-341. DOI: 10.3969/j.issn.1004-3845.2010.04.011 |
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| 作者姓名： | 石心泉 贾孟春 刘美玲 |
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| 作者单位： | 国家人口计生委科学技术研究所,北京,100081 |
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| 基金项目： | 中央级公益性科研院所基本科研业务费专项资助，国家自然科学基金资助 |
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| 摘 要： | 目的 研究LM23基因敲低后生精细胞的凋亡状况及与凋亡相关基因表达改变.方法 用TUNEL方法检测睾丸细胞的凋亡情况,用大鼠全基因组表达谱芯片检测LM23基因敲低后凋亡相关基因的表达改变.结果 TUNEL结果显示,LM23基因敲低侧大鼠睾丸生精小管内大量生精细胞发生凋亡.大鼠全基因组表达谱芯片分析结果显示,许多促凋亡基因如Bcl-2家族的促凋亡基因表达上调,而抗凋亡基因如Faf1和Zfp91基因的表达明显下调.结论 敲低大鼠的LM23基因,启动了Bcl-2家族介导的线粒体凋亡途径,引发了生精细胞发生大量凋亡.
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| 关 键 词： | LM23基因 生精细胞 细胞凋亡 Caspase-3 |
LM23-knockdown induced the apoptosis of spermatogenic cell in rat testis |
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| SHI Xin-quan,JIA Meng-chun,LIU Mei-ling. LM23-knockdown induced the apoptosis of spermatogenic cell in rat testis[J]. Journal of Reproductive Medicine, 2010, 19(4): 337-341. DOI: 10.3969/j.issn.1004-3845.2010.04.011 |
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| Authors: | SHI Xin-quan JIA Meng-chun LIU Mei-ling |
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| Affiliation: | ( National Research Institute for Family Planning, Beijing 100081) |
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| Abstract: | Objective： To assess the apoptosis of spermatogenic cell and the expressions of genes related to apoptosis in LM23-knockdown rats. Methods： TUNEL assay was used to assess the apoptosis of spermatogenic cell. The expressions of genes related to apoptosis were surveyed by microarray analysis using Agilent rat whole genome arrays in LM23-knockdown rat testis. Results： A TUNEL assay showed the presence of many apoptotic cells in seminiferous tubules. Mi- eroarray analysis found that many pro-apoptotic genes were up-regulated such as Bcl-2 family members ineluding Bax, Bid3 and Bakl. And many anti-apoptotic genes were down regulated such as Fafl and Zfp9. Conclusions： LM23-knockdown started up Bcl-2 family mediated mitochondrial-apoptosis pathway, consequently induced the apoptosis of spermatogonial cells in rat testis. |
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| Keywords: | Caspase-3 |
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