Isolation and Identification of Naegleria Species from Environmental Water in Changchun,Northeastern China |
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Authors: | Wei WANG Feng WEI Jiping LI Nan LI Quan LIU |
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Institution: | 1.Military Veterinary Institute, Academy of Military Medical Sciences, Changchun, China;2.College of Life Science, Jilin Agricultural University, Changchun, China |
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Abstract: | BackgroundNaegleria is a free-living amoeba, and pathogenic Naegleria may pose a health risk to people exposed to recreational water. Our objective in this study was to determine if there are pathogenic amoebae in environmental water samples from Changchun, Northeastern China.MethodsDuring July to September 2012, a total of 70 water samples were collected from Changchun, Northeastern China, and Naegleria was enriched by in vitro culture and detected by PCR using Naegleria genus-specific primers. Resulting PCR products were sequenced and phylogenetically analyzed to identify Naegleria species.ResultsNaegleria was detected in 65 (92.9%) of 70 water samples. DNA sequence and phylogenetic analyses based on the internal transcribed spacer (ITS) rDNA sequences revealed four Naegleria species, including N. pagei (n = 24) and N. Australiensis (n = 18), N. clarki (n = 13) and N. gruberi (n = 10), in which N. australiensis is pathogenic to mice. But the pathogenic species N. fowleri was not detected.ConclusionThis is the first report on Naegleria species in Northeastern China, showing that almost all environmental water samples were contaminated with Naegleria, including N. pagei, N. Australiensis, N. clarki and N. gruberi, which should be considered a potential public health threat. |
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Keywords: | Naegleria PCR Identification Changchun China |
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