| 铁死亡抑制剂通过保护膜铁转运蛋白减轻脓毒症相关急性肾损伤 |
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| 引用本文： | 张楷齐,吴晶魁,姜娜,林其圣,祝旭颖,李佳琳,邵兴华,杨渊婷,倪兆慧.铁死亡抑制剂通过保护膜铁转运蛋白减轻脓毒症相关急性肾损伤[J].中国临床医学,2023,30(2):229-237. |
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| 作者姓名： | 张楷齐 吴晶魁 姜娜 林其圣 祝旭颖 李佳琳 邵兴华 杨渊婷 倪兆慧 |
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| 作者单位： | 上海交通大学医学院附属仁济医院肾脏科，分子细胞(肾病)实验室，上海交通大学医学院尿毒症诊治中心，上海市腹膜透析研究中心,上海中医药大学附属曙光医院,上海交通大学医学院附属仁济医院肾脏科，分子细胞(肾病)实验室，上海交通大学医学院尿毒症诊治中心，上海市腹膜透析研究中心,上海交通大学医学院附属仁济医院肾脏科，分子细胞(肾病)实验室，上海交通大学医学院尿毒症诊治中心，上海市腹膜透析研究中心,上海交通大学医学院附属仁济医院肾脏科，分子细胞(肾病)实验室，上海交通大学医学院尿毒症诊治中心，上海市腹膜透析研究中心,上海交通大学医学院附属仁济医院肾脏科，分子细胞(肾病)实验室，上海交通大学医学院尿毒症诊治中心，上海市腹膜透析研究中心,上海交通大学医学院附属仁济医院肾脏科，分子细胞(肾病)实验室，上海交通大学医学院尿毒症诊治中心，上海市腹膜透析研究中心,上海交通大学医学院附属仁济医院肾脏科，分子细胞(肾病)实验室，上海交通大学医学院尿毒症诊治中心，上海市腹膜透析研究中心,上海交通大学医学院附属仁济医院肾脏科，分子细胞(肾病)实验室，上海交通大学医学院尿毒症诊治中心，上海市腹膜透析研究中心 |
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| 基金项目： | 国家自然科学基金项目（面上项目） |
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| 摘 要： | 目的 利用脂多糖（lipopolysaccharide, LPS）构建脓毒症体内外模型，探讨铁死亡抑制剂在脓毒症相关急性肾损伤（sepsis associated acute kidney injury, S-AKI）中的保护作用及可能机制。方法 构建脓毒症小鼠模型，随机分为4组：对照组、LPS组、LPS+铁死亡抑制剂1(Ferrostatin-1, Fer-1)组和LPS+铁死亡抑制剂2(Deferoxamine, DFO)组，每组3只小鼠。LPS组小鼠腹腔注射LPS(15 mg/kg)，铁死亡抑制剂组分别在LPS注射前30 min腹腔注射Fer-1(5 mg/kg)和DFO(100 mg/kg)。24 h后留取小鼠血液和肾脏标本进行肾功能和铁死亡相关标志物检测。体外培养人肾小管上皮细胞（HK-2），检测100μg/mL LPS刺激后细胞活性和不稳定铁池、脂质过氧化变化情况。使用siRNA转染HK-2细胞敲低膜铁转运蛋白（ferroportin, FPN）后检测铁死亡标志物变化情况。结果 与对照组相比，LPS组小鼠血肌酐、尿素氮明显升高，肾脏病理损伤加重，铁死亡标志物显著增加，而铁死...
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| 关 键 词： | 铁死亡 脓毒症 急性肾损伤 膜铁转运蛋白 铁调素 |
| 收稿时间： | 2023/2/23 0:00:00 |
| 修稿时间： | 2023/3/24 0:00:00 |
Ferroptosis inhibitors attenuate sepsis associated acute kidney injury by protecting ferroportin |
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| ZHANG Kai-qi,WU Jing-kui,JIANG N,LIN Qi-sheng,ZHU Xu-ying,LI Jia-lin,SHAO Xing-hu,YANG Yuan-ting,NI Zhao-hui.Ferroptosis inhibitors attenuate sepsis associated acute kidney injury by protecting ferroportin[J].Chinese Journal Of Clinical Medicine,2023,30(2):229-237. |
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| Authors: | ZHANG Kai-qi WU Jing-kui JIANG N LIN Qi-sheng ZHU Xu-ying LI Jia-lin SHAO Xing-hu YANG Yuan-ting NI Zhao-hui |
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| Institution: | Department of Nephrology, Molecular Cell Lab for Kidney Disease, Shanghai Peritoneal Dialysis Research Center, Ren Ji Hospital, Uremia Diagnosis and Treatment Center, Shanghai Jiao Tong University School of Medicine,Shuguang Hospital Affiliated to Shanghai University of Traditional Chinese Medicine,Department of Nephrology, Molecular Cell Lab for Kidney Disease, Shanghai Peritoneal Dialysis Research Center, Ren Ji Hospital, Uremia Diagnosis and Treatment Center, Shanghai Jiao Tong University School of Medicine,Department of Nephrology, Molecular Cell Lab for Kidney Disease, Shanghai Peritoneal Dialysis Research Center, Ren Ji Hospital, Uremia Diagnosis and Treatment Center, Shanghai Jiao Tong University School of Medicine,Department of Nephrology, Molecular Cell Lab for Kidney Disease, Shanghai Peritoneal Dialysis Research Center, Ren Ji Hospital, Uremia Diagnosis and Treatment Center, Shanghai Jiao Tong University School of Medicine,Department of Nephrology, Molecular Cell Lab for Kidney Disease, Shanghai Peritoneal Dialysis Research Center, Ren Ji Hospital, Uremia Diagnosis and Treatment Center, Shanghai Jiao Tong University School of Medicine,Department of Nephrology, Molecular Cell Lab for Kidney Disease, Shanghai Peritoneal Dialysis Research Center, Ren Ji Hospital, Uremia Diagnosis and Treatment Center, Shanghai Jiao Tong University School of Medicine,Department of Nephrology, Molecular Cell Lab for Kidney Disease, Shanghai Peritoneal Dialysis Research Center, Ren Ji Hospital, Uremia Diagnosis and Treatment Center, Shanghai Jiao Tong University School of Medicine,Department of Nephrology, Molecular Cell Lab for Kidney Disease, Shanghai Peritoneal Dialysis Research Center, Ren Ji Hospital, Uremia Diagnosis and Treatment Center, Shanghai Jiao Tong University School of Medicine |
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| Abstract: | |
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| Keywords: | ferroptosis sepsis acute kidney injury ferroportin hepcidin |
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