| cripto-1基因siRNA对乳腺癌细胞侵袭力的影响 |
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| 引用本文： | 邱志远,范钰,王崇强,黄卫兵. cripto-1基因siRNA对乳腺癌细胞侵袭力的影响[J]. 山东医药, 2010, 50(2): 27-29 |
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| 作者姓名： | 邱志远 范钰 王崇强 黄卫兵 |
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| 作者单位： | 江苏大学附属人民医院,江苏镇江,212002 |
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| 摘 要： | 目的观察cripto-1基因小于扰RNA（siRNA）对乳腺癌细胞侵袭力的影响。方法将人乳腺癌MDA—MIM68细胞分为4组，A组不处理，B组转染空载脂质体，C组转染错配siRNA，D组转染分别转染含3．125、6．25、12．5nmol／Lcfipto-1基因siRNA的脂质体。分别于处理24．48、72h收集各组细胞。采用实时定量RT—PCR法和Western blot法检测MDA—MB468的cripto-1 mRNA和蛋白，采用软琼脂集落培养试验和Boyden小室检测MDA—MB468的锚着不依赖性增殖和侵袭能力。结果与A、B、C组比较，D组MDA—MB468的cripto-1 mRNA、cripto-1蛋白、集落形成数量、穿膜细胞数均呈浓度依赖性减少（P均〈0．005）。结论cripto-1基因siRNA可抑制乳腺癌细胞侵袭。
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| 关 键 词： | 乳腺肿瘤 表皮生长因子 侵袭力 RNA干扰技术 小干扰RNA |
Effects of cripto-1 siRNA on invasion ability in human breast cancer cell |
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| QIU Zhi-yuan,FAN Yu,WANG Chong-qiang,HUANG Wei-bing. Effects of cripto-1 siRNA on invasion ability in human breast cancer cell[J]. Shandong Medical Journal, 2010, 50(2): 27-29 |
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| Authors: | QIU Zhi-yuan FAN Yu WANG Chong-qiang HUANG Wei-bing |
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| Affiliation: | QIU Zhi-yuan,FAN Yu,WANG Chong-qiang,HUANG Wei-bing(Affiliated People's Hospital of Jiangsu University,Zhenjiang 212002,P.R.China) |
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| Abstract: | Objective Toinvestigute the effect of cripto-1 small interfering RNA(siRNA)on invasion of human breast cancer cell.Methods After human breast cancer cell line MDA-MB468 were transfected by cripto-1 siRNA,the mRNA and protein of cripto-1 gene were detected by real time RT-PCR and Western blot assay,respectively.The anchorage-independent growth was exmined by colon formation in soft agar,and invasion ability was evaluated by boyden chamber model.Results The siRNA could down-regulate the level of mRNA and prot... |
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| Keywords: | beast neoplasm epidermal growth factor invasion ability RNA interference small interfering RNA |
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