Pharmacological characterization of release-regulating serotonin autoreceptors in rat cerebellum |
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| Authors: | G Bonanno G Maura M Raiteri |
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| Affiliation: | 1. National Risk Management Research Laboratory, Office of Research and Development, United States Environmental Protection Agency, Cincinnati, OH 45268, USA;2. Departments of Biology and Earth & Environment, Boston University, Boston, MA 02215, USA;3. Pegasus Technical Services, U.S. EPA, 26 W. Martin Luther King Dr. Cincinnati, OH 45268, USA;4. Metropolitan Sewer District of Greater Cincinnati, 1600 Gest St, Cincinnati, OH 45204, USA |
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| Abstract: | The release of [3H]5-hydroxytryptamine ([3H]5-HT) evoked by 15 mM KCl in superfused rat cerebellum synaptosomes was inhibited by 5-HT (pEC30 = 8.73). Methiothepin antagonized 5-HT (pA2 = 9.28); ketanserin, methysergide, cinanserin and spiperone were ineffective. The receptors involved were activated (pEC30 = 8.90) by the 5-HT1 agonist 5-methoxy-3-[1,2,3,6-tetrahydropyridin-4-yl]-1H-indole (RU 24969) X (-)Propranolol shifted to the right (pA2 = 8.05) the dose-response curve of 5-HT. The 5-HT1A agonist 8-hydroxy-2-(di-n-propylamino)tetralin (8-OH-DPAT) was ineffective. In conclusion, autoreceptors are present on 5-HT nerve endings in rat cerebellum and appear to belong to the 5-HT1B subtype. |
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