A comparative study on the diagnostic sensitivity of rodent sperm and embryos in the detection of endotoxin in earle's balanced salt solution |
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| Authors: | A. D. Esterhuizen E. Bosman A. D. E. Botes O. A. Groenewald M. V. K. Giesteira G. P. J. Labuschagne H. W. Lindeque F. A. Rodriques J. Janse Van Rensburg J. A. M. Van Schouwenburg |
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| Affiliation: | (1) Reproductive Biology Unit, Garden City Clinic, P.O. Box 96421, 2091 Brixton, South Africa;(2) Medfem Clinic, Cramerview, P.O. Box 2263, 2021 Sandton, South Africa |
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| Abstract: | Purpose The bioassay used by most IVF units to assess culture media is the mouse embryo test. The limitations of this assay are well known. The objective of this study was therefore to evaluate the in vitro bioassay potential of rodent sperm, in terms of relative sensitivities to endotoxins, and to compare the results with the routine mouse embryo and human sperm tests.Results The greater sensitivity of rodent sperm than of mouse embryos was evident in this study. A further advantage in using the mouse sperm test was the time (4–6 hr) in which endotoxins could be detected.Conclusion This rapid sperm test proved to be inexpensive, convenient, and invaluable for detecting potential sources of cytotoxicity. |
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| Keywords: | bioassay endotoxins mouse embryo test murine sperm test |
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