An16-resilin: An advanced multi-stimuli-responsive resilin-mimetic protein polymer |
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Authors: | Rajkamal Balu Naba K Dutta Namita R Choudhury Christopher M Elvin Russell E Lyons Robert Knott Anita J Hill |
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Institution: | 1. Ian Wark Research Institute, University of South Australia, Mawson Lakes, SA 5095, Australia;2. CSIRO Agriculture, Level 6, Queensland Bioscience Precinct, St Lucia, Queensland 4067, Australia;3. ANSTO, Private Mail Bag, Kirrawee, New South Wales 2232, Australia;4. CSIRO Manufacturing, Clayton, Victoria 3168, Australia |
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Abstract: | Engineered protein polymers that display responsiveness to multiple stimuli are emerging as a promising class of soft material with unprecedented functionality. The remarkable advancement in genetic engineering and biosynthesis has created the opportunity for precise control over the amino acid sequence, size, structure and resulting functions of such biomimetic proteins. Herein, we describe the multi-stimuli-responsive characteristics of a resilin-mimetic protein, An16-resilin (An16), derived from the consensus sequence of resilin gene in the mosquito Anopheles gambiae. We demonstrate that An16 is an intrinsically disordered protein that displays unusual dual-phase thermal transition behavior along with responsiveness to pH, ion, light and humidity. Identifying the molecular mechanisms that allow An16 to sense and switch in response to varying environments furthers the ability to design intelligent biomacromolecules. |
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Keywords: | Biomimetic protein An16-resilin Intrinsically disordered protein (IDP) Self-assembly Multi-stimuli responsiveness |
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