许旺细胞源神经营养因子的亚细胞定位及初步分布

目的 观察许旺细胞源神经营养因子的超微结构定位，探索其在神经、皮肤和肌肉中的分布以及神经损伤前后量的变化。方法 （1）胶体金标记免疫电镜法对预损伤2周大鼠坐骨神经超薄切片进行许旺细胞源神经营养因子的亚细胞定位；（2）免疫金银染色法对正常及损伤2周后的坐骨神经、肌肉和皮肤进行许旺细胞源神经营养因子的标记并观察量的变化。结果 （1）在许旺细胞胸浆中有明显的均匀圆形的致密金颗粒附着，但大量存在于内质钢和高尔基体上；（2）对照组、正常及失神经支配肌肉组为（－），正常神经组为（＋），正常皮肤组为（＋＋），变性神经组和失神经支配皮肤组为（＋＋＋）。结论 （1）许旺细胞中，内质网可合成许旺细胞源神经营养因子，并且在高尔基体加工；（2）神经和皮肤中存在有许旺细胞源神经营养因子表达。正常皮肤中许旺细胞源神经营养因子含量高于正常神经，失神经支配皮肤中许旺细胞源神经营养因子含量相当于变性神经。

Study on distribution and sub cell l ocat ion of Schwanncell derived neurotrophic factor

（1）To study the ultr a structure localization of Schwann cell （SC） derived neurotrophic factor （SDNF） in Schwann cells.（2）To investigate the distribution and quantitative changes in nerve、skin and muscle before injuries and after injuries. Methods （1）Fo ur SD rats were transected one side of sciatic nerve，the distal degenerative nerve were obtained after two weeks and made to ultra slices.After labeled by colloidal gold，the ultra slices were observed under tran smitted electromicroscope.（2）One side of sciatic nerve of ten SD rats were tran sected，after two weeks，both of sciatic nerves、gastrocnemius and dorsum pedis skins，no matter injured or uninjured，were obtained and made to frozen sections.After treated with immunogold-silver stain （ IGSS），the slices were observed under microscope. Results （1）There were obvious high electro-density gold-granules in the cytoplasm of the SC，but the most on the endoplasmic reticulum and Golgi apparatus.（2）There were silver staining in nerve and skin，but not in muscle. Conclusions （1）SDNF can be synthesized in the endopl asmic reticulum and processed in the Gol giapparatus of SC.（2）SDNF can be found in the normal and denerved skin，but not in the normal and denerved muscle.