藏药亚吉玛(长梗金腰)药材质量标准研究

目的 依据中国药典2020版四部通则，对藏药亚吉玛(长梗金腰)进行系统研究，建立其药材质量标准。方法 采用显微鉴别法对药材的粉末特征进行描述；采用硅胶GF254薄层板，以5-O-去甲基阿朴黄酮和羟基阿亚黄素A为对照品，建立薄层色谱鉴别方法；参照四部通则方法检测长梗金腰药材中的水分、总灰分、酸不溶性灰分及醇溶性浸出物含量；采用HPLC，建立长梗金腰药材的特征图谱，并以chrysosplenoside I(CI)和chrysosplenoside A(CA)为质控指标成分建立长梗金腰药材的含量测定方法。结果 多产地10批次样品的测定结果为水分9.17%～12.52%，总灰分14.11%～16.74%，酸不溶性灰分1.50%～4.72%，醇溶性浸出物32.77%～40.30%,CI质量分数0.30%～0.99%,CA质量分数0.28%～0.88%。结论 本研究首次建立了亚吉玛(长梗金腰)的药材鉴别和含量测定方法。该方法易于操作，专属性较强，对准确评价药材内在质量，保证用药品质具有重要意义。

Quality Standard of Tibetan Medicine "Yajima" (Chrysosplenium Axillare)

OBJECTIVE To establish the quality standards of medicinal materials in light of related methods in the general principles of part four of Chinese Pharmacopoeia(2020 Edition), and to conduct systematic research on the Tibetan medicine "Yajima"(Chrysosplenium axillare). METHODS The powder characteristics of medicinal materials were described by microscopic identification method. Silica gel GF₂₅₄ thin-layer plate was employed to establish a TLC identification method with 5-O-demethylapulein and oxyayanin A as reference substances. Loss on drying, total ash, acid-insoluble ash and ethanol-soluble extractives of 10 batches of Chrysosplenium axillare were determined according to the general principles of part four of Chinese Pharmacopoeia(2020 Edition). HPLC was used to establish the characteristic chromatogram of Chrysosplenium axillare, and the content determination method was established with chrysosplenoside I(CI) and chrysosplenoside A(CA) as the quality control index components of Chrysosplenium axillare. RESULTS The water content, total ash, acid-insoluble ash, ethanol-soluble extractive and the content of CI and CA of all samples varied in the ranges of 9.17%?12.52%, 14.11%?16.74%, 1.50%?4.72%, 32.77%?40.30%, 0.30%?0.99% and 0.28%?0.88%, respectively. CONCLUSION The identification and content determination methods of Yajima(Chrysosplenium axillare) are established for the first time. The methods are easy to operate and exclusive, which is of great significance to accurately evaluate the internal quality of medicinal materials and ensure the quality of drug used.