游离脂肪酸诱导3T3-L1细胞胰岛素抵抗模型的建立

目的观察不同种类和浓度游离脂肪酸(FFA)对3T3-L1细胞葡萄糖摄取及胰岛素敏感性的影响,建立FFA诱导的胰岛素抵抗细胞模型。方法3T3-L1细胞体外诱导分化为脂肪细胞,油红O染色鉴定,不同浓度软脂酸(PA)和油酸(OA)诱导,测定基础状态和胰岛素刺激下葡萄糖特异性转运情况。结果3T3-L1脂肪细胞诱导率为98%±1.3%,PA和OA各组胰岛素刺激后的葡萄糖特异性转运呈时间和浓度依赖性下降趋势,均显著低于对照组(p<0.01);0.5mM PA作用24h后基础葡萄糖特异性转运明显低于对照组和PA 0.25mM作用24h组(p<0.05)。结论3T3-L1细胞在体外可稳定分化为成熟脂肪细胞,经FFA诱导成为胰岛素抵抗细胞模型;随FFA作用时间延长和浓度增加,胰岛素抵抗程度加重,并以时间和浓度依赖性方式抑制3T3-L1脂肪细胞胰岛素刺激后的葡萄糖特异性转运,高浓度时抑制基础状态下的葡萄糖特异性转运。

The Establishment of 3T3-L1 Insulin Resistance Cell Model by Free Fatty Acids Induction

Objective To observe the influence of free fatty acids(FFA)on glucose uptake and insulin sensitivity of 3T3-L1 adipocytes and establish the FFA-induced insulin resistance cell model.Methods 3T3-L1 cells were induced into adipocytes identified by oil red O staining,specific glucose transport was determined in different concentrations of palmitic acid(PA)and oleic acid(OA)induction.Results 3T3-L1 adipocytes induction rate averaged 98%±1.3% by Student method.Specific glucose transport by PA and OA appeared a time and concentration-dependent downward trend after insulin stimulation,significantly lower than the control(p<0.01).Specific glucose transport of the basic state decreased significantly 24h after 0.5 mM PA induction compared with the control and 0.25 mM PA induction for 24h(p<0.05). Conclusion 3T3-L1 cell can be induced into mature fat cells in vitro,the FFA-induced 3T3-L1 adipocytes produce insulin resistance cell model.Insulin-stimulated specific glucose transport was inhibited in a concentration and time- dependent manner,while that of the basic state was inhibited at high concentration FFA induction.