| 以基因芯片技术研究Kkay小鼠2型糖尿病相关基因 |
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| 引用本文： | Guo QH,Lu JM,Yin L,Pan CY. 以基因芯片技术研究Kkay小鼠2型糖尿病相关基因[J]. 中国医学科学院学报, 2002, 24(5): 462-465 |
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| 作者姓名： | Guo QH Lu JM Yin L Pan CY |
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| 作者单位： | 1. 中国人民解放军总医院内分泌科,北京,100853
2. Neuroinformatics Center,General Hospital of PLA,Beijing,100853 |
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| 摘 要： | 目的以基因芯片技术研究Kkay2型糖尿病小鼠的差异表达基因。方法以包含8192条小鼠的cDNA基因表达谱芯片研究Kkay2型糖尿病小鼠肝脏的基因表达谱。结果共筛选出差异表达基因154条,包括全长基因68条,表达序列标识(EST)86条;其中40条基因表达增加,114条表达降低。结论多数已知功能基因的表达异常与以往的研究结论是一致的;cDNA基因芯片技术能够高通量分析糖尿病相关基因。
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| 关 键 词： | 基因芯片 微矩阵 基因表达谱 糖尿病 Kkay小鼠 |
| 修稿时间： | 2002-03-15 |
Study of the gene expression pattern in the Kkay mouse with type 2 diabetes by genomic-scale gene expression analysis |
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| Guo Qing-hua,Lu Ju-ming,Yin Ling,Pan Chang-yu. Study of the gene expression pattern in the Kkay mouse with type 2 diabetes by genomic-scale gene expression analysis[J]. Acta Academiae Medicinae Sinicae, 2002, 24(5): 462-465 |
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| Authors: | Guo Qing-hua Lu Ju-ming Yin Ling Pan Chang-yu |
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| Affiliation: | Endocrinology Department, General Hospital of PLA, Beijing 100853, China. |
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| Abstract: | Objective To study the genes differentially expressed in the liver of Kkay diabetic and normal mice by genomic-scale gene expression analysis.Methods cDNA microarray chips containing8192cDNAs were used to explore the gene expression pattern of Kkay mouse liver.Results One hundred and fifty-four genes were screened out,including68complete cDNAs and expressed sequence tags,and among them40genes were up-regulated and114genes were down-regulated respectively.Conclusion Most of the gene expression analysis results were consistent with previous study,and the gene expression pattern of Kkay mouse based on cDNA microarray could be used for high-throughout screening out the genes associated with type2diabetes. |
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| Keywords: | gene chip cDNA microarray gene expression pattern diabetes mellitus Kkay mouse |
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