二烯丙基二硫下调p38活性抑制乳腺癌MCF-7细胞侵袭和转移

目的探讨大蒜提取物二烯丙基二硫(DADS)对乳腺癌MCF-7 细胞侵袭转移的作用及其机制。方法采用不同浓度（0， 100，200，400 μmol/L）DADS处理MCF-7人乳腺癌细胞24 h，Transwell侵袭实验检测DADS对细胞侵袭的作用，划痕愈合实验 观察DADS对细胞迁移的改变，Western blotting 检测细胞中上皮标志蛋白E-钙黏蛋白（E-cadherin）、间质标志蛋白波形蛋白 （Vimentin）和基质金属蛋白MMP-9的表达和p38活性；TGF-β1上调p-p38表达后上述作用的变化。结果Transwell和划痕愈 合实验发现DADS呈浓度依赖性抑制MCF-7 细胞的侵袭和迁移能力，Western blotting 结果表明DADS可抑制Vimentin 和 MMP-9 的蛋白表达，上调E-cadherin 的表达而下调p38 活性；TGF-β1 可上调p-p38 和MMP-9 表达，明显抵消DADS对MCF-7 细胞的抑制效果。结论DADS可下调p38活性，抑制MCF-7细胞的侵袭转移。

Diallyl disulfide inhibits invasion and metastasis of MCF-7 breast cancer cells in vitro by down-regulating p38 activity

Objective To investigate the effect of diallyl disulfide (DADS) on invasion and metastasis of human breast cancer MCF-7 cells and explore the possible mechanism. Methods MCF-7 cells treated with 100, 200, and 400μmol/L of DADS for 24 h were examined for cell invasion and migration capacities using Transwell assay and wound healing assay, respectively. The protein expression of E-cadherin, vimentin, MMP-9 and p-p38 in the cells were detected with Western blotting. The effect of transforming growth factor-β1 (TGF-β1) as the agonist of p38 activity was tested in antagonizing the effects of DADS. Results DADS inhibited the invasion and migration of MCF-7 cells in a dose-dependent manner, down-regulated the protein expression of Vimentin and MMP-9 and up-regulated E-cadherin expression in the cells. Treatment with TGF-β1 to up-regulate p38 activity obviously antagonized the inhibitory effect of DADS on the invasion and metastasis of MCF-7 cells. Conclusion DADS can inhibit the invasion and metastasis of MCF-7 cells in vitro by down-regulating p38 activity.