二烯丙基二硫下调p38活性抑制乳腺癌MCF-7细胞侵袭和转移 |
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引用本文: | 陈霄霄,刘晓旺,周志刚,陈雪艳,李丽丁,熊婷,彭露,涂剑. 二烯丙基二硫下调p38活性抑制乳腺癌MCF-7细胞侵袭和转移[J]. 南方医科大学学报, 2016, 36(6): 814-818. DOI: 10.3969/j.issn.1673-4254.2016.06.14 |
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作者姓名: | 陈霄霄 刘晓旺 周志刚 陈雪艳 李丽丁 熊婷 彭露 涂剑 |
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作者单位: | 1. 南华大学 药物药理研究所,湖南 衡阳,421001;2. 南华大学 附属第一医院,湖南 衡阳,421001 |
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基金项目: | 国家自然科学基金(81541163);国家级大学生创新创业训练计划项目(201410555008);湖南省大学生创新性课题(2014-234);湖南省中医药局课题(2014144);南华大学研究生创新性课题(2015XCX33),“湖南省分子靶标新药研究协同创新中心”培育项目(2014-405) Supported by National Natural Science Foundation of China(81541163) |
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摘 要: | 目的探讨大蒜提取物二烯丙基二硫(DADS)对乳腺癌MCF-7 细胞侵袭转移的作用及其机制。方法采用不同浓度(0,100,200,400 μmol/L)DADS处理MCF-7人乳腺癌细胞24 h,Transwell侵袭实验检测DADS对细胞侵袭的作用,划痕愈合实验观察DADS对细胞迁移的改变,Western blotting 检测细胞中上皮标志蛋白E-钙黏蛋白(E-cadherin)、间质标志蛋白波形蛋白(Vimentin)和基质金属蛋白MMP-9的表达和p38活性;TGF-β1上调p-p38表达后上述作用的变化。结果Transwell和划痕愈合实验发现DADS呈浓度依赖性抑制MCF-7 细胞的侵袭和迁移能力,Western blotting 结果表明DADS可抑制Vimentin 和MMP-9 的蛋白表达,上调E-cadherin 的表达而下调p38 活性;TGF-β1 可上调p-p38 和MMP-9 表达,明显抵消DADS对MCF-7细胞的抑制效果。结论DADS可下调p38活性,抑制MCF-7细胞的侵袭转移。
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关 键 词: | 二烯丙基二硫(DADS) 乳腺癌MCF-7细胞 p38活性 侵袭 转移 |
Diallyl disulfide inhibits invasion and metastasis of MCF-7 breast cancer cells in vitro by down-regulating p38 activity |
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Abstract: | Objective To investigate the effect of diallyl disulfide (DADS) on invasion and metastasis of human breast cancer MCF-7 cells and explore the possible mechanism. Methods MCF-7 cells treated with 100, 200, and 400μmol/L of DADS for 24 h were examined for cell invasion and migration capacities using Transwell assay and wound healing assay, respectively. The protein expression of E-cadherin, vimentin, MMP-9 and p-p38 in the cells were detected with Western blotting. The effect of transforming growth factor-β1 (TGF-β1) as the agonist of p38 activity was tested in antagonizing the effects of DADS. Results DADS inhibited the invasion and migration of MCF-7 cells in a dose-dependent manner, down-regulated the protein expression of Vimentin and MMP-9 and up-regulated E-cadherin expression in the cells. Treatment with TGF-β1 to up-regulate p38 activity obviously antagonized the inhibitory effect of DADS on the invasion and metastasis of MCF-7 cells. Conclusion DADS can inhibit the invasion and metastasis of MCF-7 cells in vitro by down-regulating p38 activity. |
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Keywords: | diallyl disulfide MCF-7 breast cancer cells p38 activity invasion metastasis |
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