| 外源性HPV16 E7癌基因表达对宫颈癌HeLa细胞Cyclin E表达的影响 |
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| 引用本文： | 田甜,戴淑真,孔守芳,黄丽丽,罗兵.外源性HPV16 E7癌基因表达对宫颈癌HeLa细胞Cyclin E表达的影响[J].青岛大学医学院学报,2007,43(3):210-211,214. |
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| 作者姓名： | 田甜 戴淑真 孔守芳 黄丽丽 罗兵 |
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| 作者单位： | 青岛大学医学院附属医院妇科,山东,青岛,266003;青岛大学医学院微生物学教研室,山东,青岛,266003 |
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| 摘 要： | 目的研究重组腺病毒介导人乳头状瘤病毒（HPV）16型E7病毒癌基因感染对人宫颈癌HeLa细胞周期蛋白E（Cyclin E）表达的影响,探讨HPV16 E7癌基因以及Cyclin E在宫颈癌发生发展中的作用。方法用含有HPV16 E7病毒癌基因的重组腺病毒载体感染体外培养的人宫颈癌HeLa细胞。免疫荧光染色后激光扫描共聚焦显微镜（LSCM）检测感染前后Cyclin E表达的差异。结果HPV16 E7感染后Cyclin E表达较感染前明显增加。结论HPV16 E7基因的表达促进Cyclin E的表达,对宫颈肿瘤细胞有增殖促进的作用。
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| 关 键 词： | 乳头状瘤病毒 人 基因 E7 感染 宫颈肿瘤 细胞周期蛋白E |
| 文章编号： | 1672-4488（2007）03-0210-03 |
| 修稿时间： | 2006-06-182006-12-15 |
INFLUENCE ON EXPRESSION OF Cyclin E IN HeLa CELLS BY EXPRESSION OF THE EXOGENOUS HPV16 E7 ONCOGENE |
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| TIAN TIAN, DAI SHU-ZHEN, KONG SHOU-FANG, et al.INFLUENCE ON EXPRESSION OF Cyclin E IN HeLa CELLS BY EXPRESSION OF THE EXOGENOUS HPV16 E7 ONCOGENE[J].Acta Academiae Medicinae Qingdao Universitatis,2007,43(3):210-211,214. |
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| Authors: | TIAN TIAN DAI SHU-ZHEN KONG SHOU-FANG |
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| Institution: | Department of Gynecology, The Affiliated Hospital of Qingdao University Medical College, Qingdao 266003, China |
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| Abstract: | ObjectiveTo investigate the expression of Cyclin E in HeLa cells after the transfection of HPV16 E7 oncogene by recombinant adenovirus vector,and assess the effect of HPV16 E7 oncogene and Cyclin E in the development of human cervical carcinoma.MethodsExpression of Cyclin E was detected by laser scanning confocal microscope(LSCM) before and after the transfection.ResultsExpression of Cyclin E protein in the HeLa cells increased significantly after the transfection of HPV16 E7 genes.ConclusionExpression of HPV16 E7 oncogene influences the expression of Cyclin E and enhances the proliferation of the cells of cervical carcinoma. |
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| Keywords: | papillomavirus human genes E7 infection cervix neoplasms Cyclin E |
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