| Hydrolysis of soy isoflavone conjugates using enzyme may underestimate isoflavone concentrations in tissue |
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| 作者姓名： | HebronC.Chang MyriamLaly MelodyHarrison ThomasM.Badger |
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| 作者单位： | [1]ArkansasChildren＇sNutritionCenter,AgriculturalResearchService,USDeparlmentofAgriculture,LittleRock,AR72202,USA [2]DepartmentofPhysiology／Biophysics,UniversityofArkansasforMedicalSciences,LittleRock,AR72202,USA [3]ArkansasChildren＇sNutritionCenter,AgriculturalResearchService,USDepartmentofAgriculture,LittleRock,AR72202,USA／／Currentaddress：InstituteofToxicology,NanjingMedicalUniversity,Nanjing210025,China |
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| 基金项目： | US Department of Agriculture/Agriculture research Service under CRIS Project number USDA ARS 6251 51000 003 06S |
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| 摘 要： | Objective: To investigate the differences of using enzymatic hydrolysis and acid hydrolysis for identification and quantification of isoflavone aglycones from biomatrices. Methods: β-glucuronidase/sulfatase isolated from Helix pomat/a for routine enzy-matic hydrolysis or 6N HC1 was used to release glucuronide and sulfate conjugates in the serum, urine and tissue samples. Profiles of soy isoflavones after enzymatic hydrolysis or acid hydrolysis in several tissues of rat fed with diets containing soy protein isolate were also com-pared using I.C/MS and HPLC-ECD. Results: Acid hydrolysis released more aglycone than enzymatic digestion (P＜0.05) in liver tissue. The total genistein, daidzein and other metabolites were 20% to 60% lower in samples from enzymatic hydrolysis than in acid hydrolysis. Conclusion: These results indicated that unknown factors in tissues reduced the enzymatic hydrolytic efficiency for releasing isoflavone aglycones even in op optimized condition. This would underestimate isoflavone tissue concentrations up to 60%.
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| 关 键 词： | 水解作用 大豆异黄酮 酶变化 糖苷配基 葡糖醛酰胺 |
| 收稿时间： | 2004/12/15 0:00:00 |
Hydrolysis of soy isoflavone conjugates using enzyme may underestimate isoflavone concentrations in tissue |
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| HebronC.Chang MyriamLaly MelodyHarrison ThomasM.Badger.Hydrolysis of soy isoflavone conjugates using enzyme may underestimate isoflavone concentrations in tissue[J].Acta Universitatis Medicinalis Nanjing,2005,19(1):10-14. |
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| Authors: | Hebron CChang Myriam Laly Melody Harrison Thomas MBadger |
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| Abstract: | Objective: To investigate the differences of using enzymatic hydrolysis and acid hydrolysis for identification and quantification of isoflavone aglycones from biomatrices. Methods: β-glucuronidase/sulfatase isolated from Helix pomatia for routine enzymatic hydrolysis or 6N HCl was used to release glucuronide and sulfate conjugates in the serum, urine and tissue samples. Profiles of soy isoflavones after enzymatic hydrolysis or acid hydrolysis in several tissues of rat fed with diets containing soy protein isolate were also compared using LC/MS and HPLC-ECD. Results: Acid hydrolysis released more aglycone than enzymatic digestion ( P ＜0.05) in liver tissue. The total genistein, daidzein and other metabolites were 20％ to 60％ lower in samples from enzymatic hydrolysis than in acid hydrolysis. Conclusion: These results indicated that unknown factors in tissues reduced the enzymatic hydrolytic efficiency for releasing isoflavone aglycones even in optimized condition. This would underestimate isoflavone tissue concentrations up to 60％. |
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| Keywords: | isoflavone aglycones enzymatic hydrolysis acid hydrolysis glucuronide sulfate conjugates |
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